Human papillomavirus type 6 long control region and human cellular DNA contain related sequences

T. F. Hrisomalos, D. L. Boggs, K. H. Fife

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have identified a region of human papillomavirus type 6 (HPV-6) DNA that hybridizes with human cellular DNA containing no detectable HPV DNA sequences. The region of hybridization has been localized to a segment of the viral long control region between the end of the L1 open reading frame and the late polyadenylation signal and is likely contained within a 94-base-pair insertion at nucleotide 7350 which is present in the cloned HPV-6b DNA used for these studies. Restriction fragments of HPV-6 DNA from seven patients suggested that this insert was present in these naturally occurring viral genomes as well. The presence of this insert was confirmed by direct sequence analysis of polymerase chain reaction-amplified segments from four naturally occurring HPV-6 genomes. By analogy with other systems, this insert and surrounding sequences may function to destabilize the HPV-6 late mRNA.

Original languageEnglish (US)
Pages (from-to)5188-5191
Number of pages4
JournalJournal of virology
Volume64
Issue number10
StatePublished - Oct 5 1990

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Human papillomavirus 6
Papillomaviridae
DNA
Polyadenylation
Viral Genome
Base Pairing
genome
Open Reading Frames
Sequence Analysis
Nucleotides
Genome
open reading frames
hybridization
Polymerase Chain Reaction
sequence analysis
Messenger RNA
polymerase chain reaction
nucleotides
nucleotide sequences

ASJC Scopus subject areas

  • Immunology

Cite this

Human papillomavirus type 6 long control region and human cellular DNA contain related sequences. / Hrisomalos, T. F.; Boggs, D. L.; Fife, K. H.

In: Journal of virology, Vol. 64, No. 10, 05.10.1990, p. 5188-5191.

Research output: Contribution to journalArticle

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