Human platelet lysate improves human cord blood derived ECFC survival and vasculogenesis in three dimensional (3D) collagen matrices

Hyojin Kim, Nutan Prasain, Sasidhar Vemula, Michael J. Ferkowicz, Momoko Yoshimoto, Sherry L. Voytik-Harbin, Mervin Yoder

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Human cord blood (CB) is enriched in circulating endothelial colony forming cells (ECFCs) that display high proliferative potential and in vivo vessel forming ability. Since diminished ECFC survival is known to dampen the vasculogenic response in vivo, we tested how long implanted ECFC survive and generate vessels in three-dimensional (3D) type I collagen matrices in vitro and in vivo. We hypothesized that human platelet lysate (HPL) would promote cell survival and enhance vasculogenesis in the 3D collagen matrices. We report that the percentage of ECFC co-cultured with HPL that were alive was significantly enhanced on days 1 and 3 post-matrix formation, compared to ECFC alone containing matrices. Also, co-culture of ECFC with HPL displayed significantly more vasculogenic activity compared to ECFC alone and expressed significantly more pro-survival molecules (pAkt, p-Bad and Bcl-xL) in the 3D collagen matrices in vitro. Treatment with Akt1 inhibitor (A-674563), Akt2 inhibitor (CCT128930) and Bcl-xL inhibitor (ABT-263/Navitoclax) significantly decreased the cell survival and vasculogenesis of ECFC co-cultured with or without HPL and implicated activation of the Akt1 pathway as the critical mediator of the HPL effect on ECFC in vitro. A significantly greater average vessel number and total vascular area of human CD31<sup>+</sup> vessels were present in implants containing ECFC and HPL, compared to the ECFC alone implants in vivo. We conclude that implantation of ECFC with HPL in vivo promotes vasculogenesis and augments blood vessel formation via diminishing apoptosis of the implanted ECFC.

Original languageEnglish
Pages (from-to)72-81
Number of pages10
JournalMicrovascular Research
Volume101
DOIs
StatePublished - Sep 1 2015

Fingerprint

Platelets
Fetal Blood
Cell Survival
Blood
Collagen
Blood Platelets
Cells
Blood Vessels
Cultured Cells
Blood vessels
Collagen Type I
Critical Pathways
Chemical activation
Coculture Techniques
Apoptosis
Molecules
Survival

Keywords

  • Apoptosis
  • Endothelial colony forming cells (ECFCs)
  • Human platelet lysate (HPL)
  • Three dimensional (3D) collagen matrix
  • Vasculogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Human platelet lysate improves human cord blood derived ECFC survival and vasculogenesis in three dimensional (3D) collagen matrices. / Kim, Hyojin; Prasain, Nutan; Vemula, Sasidhar; Ferkowicz, Michael J.; Yoshimoto, Momoko; Voytik-Harbin, Sherry L.; Yoder, Mervin.

In: Microvascular Research, Vol. 101, 01.09.2015, p. 72-81.

Research output: Contribution to journalArticle

Kim, Hyojin ; Prasain, Nutan ; Vemula, Sasidhar ; Ferkowicz, Michael J. ; Yoshimoto, Momoko ; Voytik-Harbin, Sherry L. ; Yoder, Mervin. / Human platelet lysate improves human cord blood derived ECFC survival and vasculogenesis in three dimensional (3D) collagen matrices. In: Microvascular Research. 2015 ; Vol. 101. pp. 72-81.
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AU - Ferkowicz, Michael J.

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