Human recombinant stem cell factor stimulates in vitro proliferation of acute myeloid leukemia cells and expands the clonogenic cell pool

Nadia Carlesso, Patrizia Pregno, Paola Bresso, Eugenio Gallo, Alessandro Pileri, Krisztina M. Zsebo, Dario Ferrero

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Stem cell factor (SCF) is a new growth factor acting on early hematopoietic progenitor and stem cells. In our experiments human recombinant SCF stimulated short-term proliferation of accessory cell-depleted acute myeloid leukemia (AML) cells in 13/14 cases, as determined by 3H-thymidine (3H-TdR) incorporation and cell counts. Stimulatory activity was significantly greater than in the presence of GM-CSF and was comparable to that of granulocyte colony-stimulating factor (G-CSF), interleukin 3 (IL-3), and 5637 cell line supernatant (SN). Conversely, the ability of SCF to induce primary colony formation by AML clonogenic cells (CFU-L) was lower than that of granulocyte-macrophage colony-stimulating factor (GM-CSF) and 5637 SN in all but four cases. However, SCF potentiated trie stimulatory effect of GM-CSF, G-CSF, and IL-3 on both 3H-TdR incorporation and colony formation. In a 7-day liquid culture SCF enhanced CFU-L recovery in all cases to a significantly greater extent than the other growth factors. A further increment was obtained by combinations of SCF with GM-CSF, G-CSF, or IL-3, and this was significantly more effective than 5637 SN. SCF did not induce leukemic cell differentiation. Human recombinant SCF is therefore highly efficient in stimulating AML cell proliferation and expanding the CFU-L pool. It was not, however, able to support long-term growth of AML cells (beyond 2-7 weeks) in five cases tested.

Original languageEnglish (US)
Pages (from-to)642-648
Number of pages7
JournalLeukemia
Volume6
Issue number7
StatePublished - Jul 1992
Externally publishedYes

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Stem Cell Factor
Myeloid Cells
Acute Myeloid Leukemia
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-3
Granulocyte Colony-Stimulating Factor
Hematopoietic Stem Cells
Intercellular Signaling Peptides and Proteins
Cell Proliferation
In Vitro Techniques
Thymidine
Cell Differentiation
Cell Count
Cell Line
Growth

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Human recombinant stem cell factor stimulates in vitro proliferation of acute myeloid leukemia cells and expands the clonogenic cell pool. / Carlesso, Nadia; Pregno, Patrizia; Bresso, Paola; Gallo, Eugenio; Pileri, Alessandro; Zsebo, Krisztina M.; Ferrero, Dario.

In: Leukemia, Vol. 6, No. 7, 07.1992, p. 642-648.

Research output: Contribution to journalArticle

Carlesso, N, Pregno, P, Bresso, P, Gallo, E, Pileri, A, Zsebo, KM & Ferrero, D 1992, 'Human recombinant stem cell factor stimulates in vitro proliferation of acute myeloid leukemia cells and expands the clonogenic cell pool', Leukemia, vol. 6, no. 7, pp. 642-648.
Carlesso, Nadia ; Pregno, Patrizia ; Bresso, Paola ; Gallo, Eugenio ; Pileri, Alessandro ; Zsebo, Krisztina M. ; Ferrero, Dario. / Human recombinant stem cell factor stimulates in vitro proliferation of acute myeloid leukemia cells and expands the clonogenic cell pool. In: Leukemia. 1992 ; Vol. 6, No. 7. pp. 642-648.
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