Human T-Cell Leukemia Virus (HTLV) Type II Rex Protein Binds Specifically to RNA Sequences of the HTLV Long Terminal Repeat but Poorly to the Human Immunodeficiency Virus Type 1 Rev-Responsive Element

Michele Yip-Schneider, William S. Dynan, Patrick L. Green, Alexander C. Black, Salvatore J. Arrigo, Arian Torbati, Shaun Heaphy, Cristina Ruland, Joseph D. Rosenblatt, Irvin S Y Chen

Research output: Contribution to journalArticle

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Abstract

The human T-cell leukemia viruses (HTLVs) encode a trans-regulatory protein, Rex, which differentially regulates viral gene expression by controlling the cytoplasmic accumulation of viral mRNAs. Because of insufficient amounts of purified protein, biochemical characterization of Rex activity has not previously been performed. Here, utilizing the baculovirus expression system, we purified HTLV type II (HTLV-II) Rex from the cytoplasmic fraction of recombinant baculovirus-infected insect cells by heparin-agarose chromatography. We directly demonstrated that Rex specifically bound HTLV-II 5′ long terminal repeat RNA in both gel mobility shift and immunobinding assays. Sequences sufficient for Rex binding were localized to the R-U5 region of the HTLV-II 5′ long terminal repeat and correlate with the region required for Rex function. The human immunodeficiency virus type 1 (HIV-1), has an analogous regulatory protein, Rev, which directly binds to and mediates its action through the Rev-responsive element located within the HIV-1 env gene. We demonstrated that HTLV-II Rex rescued an HIV-1JR-CSF Rev-deficient mutant, although inefficiently. This result is consistent with a weak binding activity to the HIV-1 Rev-responsive element under conditions in which it efficiently bound the HTLV-II long terminal repeat RNA.

Original languageEnglish (US)
Pages (from-to)2261-2272
Number of pages12
JournalJournal of Virology
Volume65
Issue number5
StatePublished - May 1991
Externally publishedYes

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rex Gene Products
Deltaretrovirus
Human T-lymphotropic virus 2
env Genes
terminal repeat sequences
Terminal Repeat Sequences
Human immunodeficiency virus 1
HIV-1
nucleotide sequences
Baculoviridae
proteins
regulatory proteins
rev Gene Products
RNA
Agarose Chromatography
Viral Genes
Electrophoretic Mobility Shift Assay
heparin
Insects
agarose

ASJC Scopus subject areas

  • Immunology

Cite this

Human T-Cell Leukemia Virus (HTLV) Type II Rex Protein Binds Specifically to RNA Sequences of the HTLV Long Terminal Repeat but Poorly to the Human Immunodeficiency Virus Type 1 Rev-Responsive Element. / Yip-Schneider, Michele; Dynan, William S.; Green, Patrick L.; Black, Alexander C.; Arrigo, Salvatore J.; Torbati, Arian; Heaphy, Shaun; Ruland, Cristina; Rosenblatt, Joseph D.; Chen, Irvin S Y.

In: Journal of Virology, Vol. 65, No. 5, 05.1991, p. 2261-2272.

Research output: Contribution to journalArticle

Yip-Schneider, Michele ; Dynan, William S. ; Green, Patrick L. ; Black, Alexander C. ; Arrigo, Salvatore J. ; Torbati, Arian ; Heaphy, Shaun ; Ruland, Cristina ; Rosenblatt, Joseph D. ; Chen, Irvin S Y. / Human T-Cell Leukemia Virus (HTLV) Type II Rex Protein Binds Specifically to RNA Sequences of the HTLV Long Terminal Repeat but Poorly to the Human Immunodeficiency Virus Type 1 Rev-Responsive Element. In: Journal of Virology. 1991 ; Vol. 65, No. 5. pp. 2261-2272.
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abstract = "The human T-cell leukemia viruses (HTLVs) encode a trans-regulatory protein, Rex, which differentially regulates viral gene expression by controlling the cytoplasmic accumulation of viral mRNAs. Because of insufficient amounts of purified protein, biochemical characterization of Rex activity has not previously been performed. Here, utilizing the baculovirus expression system, we purified HTLV type II (HTLV-II) Rex from the cytoplasmic fraction of recombinant baculovirus-infected insect cells by heparin-agarose chromatography. We directly demonstrated that Rex specifically bound HTLV-II 5′ long terminal repeat RNA in both gel mobility shift and immunobinding assays. Sequences sufficient for Rex binding were localized to the R-U5 region of the HTLV-II 5′ long terminal repeat and correlate with the region required for Rex function. The human immunodeficiency virus type 1 (HIV-1), has an analogous regulatory protein, Rev, which directly binds to and mediates its action through the Rev-responsive element located within the HIV-1 env gene. We demonstrated that HTLV-II Rex rescued an HIV-1JR-CSF Rev-deficient mutant, although inefficiently. This result is consistent with a weak binding activity to the HIV-1 Rev-responsive element under conditions in which it efficiently bound the HTLV-II long terminal repeat RNA.",
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