Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure

Alexander C. Black, Cristina T. Ruland, Michele Yip-Schneider, Jie Luo, Bich Tran, Anupama Kalsi, Eddie Quan, Mordecai Aboud, Irvin S Y Chen, Joseph D. Rosenblatt

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The human T-cell leukemia virus type II (HTLV-II) regulatory protein Rex augments cytoplasmic levels of unspliced gag-pol mRNA by acting through a Rex-responsive element (RxRE) in the long terminal repeat. Purified Rex protein binds to long terminal repeat mRNA. Here, using an immunobinding assay to measure the binding of Rex protein to mutated RxRE RNAs, we show that efficient Rex binding requires a stem-bulge-loop RNA secondary structure (nucleotides [nt] 465 to 500) and specific sequences both within the stem-bulge (nt 470 to 476) and within a conserved upstream splice donor site (nt 449 to 455). Rex function in a transient transfection expression system correlates with Rex protein-RxRE RNA binding. The ability of HTLV-II Rex to interact directly with the HTLV-II splice donor site suggests that HTLV-II Rex may increase expression of unspliced gag-pol mRNA, in part, by inhibiting splicing.

Original languageEnglish (US)
Pages (from-to)6645-6653
Number of pages9
JournalJournal of Virology
Volume65
Issue number12
StatePublished - Dec 1991
Externally publishedYes

Fingerprint

rex Gene Products
Deltaretrovirus
Human T-lymphotropic virus 2
RNA Splice Sites
RNA
terminal repeat sequences
Nucleotides
Terminal Repeat Sequences
nucleotides
Messenger RNA
stems
proteins
regulatory proteins
transfection
Transfection
Carrier Proteins
assays

ASJC Scopus subject areas

  • Immunology

Cite this

Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure. / Black, Alexander C.; Ruland, Cristina T.; Yip-Schneider, Michele; Luo, Jie; Tran, Bich; Kalsi, Anupama; Quan, Eddie; Aboud, Mordecai; Chen, Irvin S Y; Rosenblatt, Joseph D.

In: Journal of Virology, Vol. 65, No. 12, 12.1991, p. 6645-6653.

Research output: Contribution to journalArticle

Black, AC, Ruland, CT, Yip-Schneider, M, Luo, J, Tran, B, Kalsi, A, Quan, E, Aboud, M, Chen, ISY & Rosenblatt, JD 1991, 'Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure', Journal of Virology, vol. 65, no. 12, pp. 6645-6653.
Black, Alexander C. ; Ruland, Cristina T. ; Yip-Schneider, Michele ; Luo, Jie ; Tran, Bich ; Kalsi, Anupama ; Quan, Eddie ; Aboud, Mordecai ; Chen, Irvin S Y ; Rosenblatt, Joseph D. / Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure. In: Journal of Virology. 1991 ; Vol. 65, No. 12. pp. 6645-6653.
@article{46e285233d3d49978aa770bb362e50db,
title = "Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure",
abstract = "The human T-cell leukemia virus type II (HTLV-II) regulatory protein Rex augments cytoplasmic levels of unspliced gag-pol mRNA by acting through a Rex-responsive element (RxRE) in the long terminal repeat. Purified Rex protein binds to long terminal repeat mRNA. Here, using an immunobinding assay to measure the binding of Rex protein to mutated RxRE RNAs, we show that efficient Rex binding requires a stem-bulge-loop RNA secondary structure (nucleotides [nt] 465 to 500) and specific sequences both within the stem-bulge (nt 470 to 476) and within a conserved upstream splice donor site (nt 449 to 455). Rex function in a transient transfection expression system correlates with Rex protein-RxRE RNA binding. The ability of HTLV-II Rex to interact directly with the HTLV-II splice donor site suggests that HTLV-II Rex may increase expression of unspliced gag-pol mRNA, in part, by inhibiting splicing.",
author = "Black, {Alexander C.} and Ruland, {Cristina T.} and Michele Yip-Schneider and Jie Luo and Bich Tran and Anupama Kalsi and Eddie Quan and Mordecai Aboud and Chen, {Irvin S Y} and Rosenblatt, {Joseph D.}",
year = "1991",
month = "12",
language = "English (US)",
volume = "65",
pages = "6645--6653",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure

AU - Black, Alexander C.

AU - Ruland, Cristina T.

AU - Yip-Schneider, Michele

AU - Luo, Jie

AU - Tran, Bich

AU - Kalsi, Anupama

AU - Quan, Eddie

AU - Aboud, Mordecai

AU - Chen, Irvin S Y

AU - Rosenblatt, Joseph D.

PY - 1991/12

Y1 - 1991/12

N2 - The human T-cell leukemia virus type II (HTLV-II) regulatory protein Rex augments cytoplasmic levels of unspliced gag-pol mRNA by acting through a Rex-responsive element (RxRE) in the long terminal repeat. Purified Rex protein binds to long terminal repeat mRNA. Here, using an immunobinding assay to measure the binding of Rex protein to mutated RxRE RNAs, we show that efficient Rex binding requires a stem-bulge-loop RNA secondary structure (nucleotides [nt] 465 to 500) and specific sequences both within the stem-bulge (nt 470 to 476) and within a conserved upstream splice donor site (nt 449 to 455). Rex function in a transient transfection expression system correlates with Rex protein-RxRE RNA binding. The ability of HTLV-II Rex to interact directly with the HTLV-II splice donor site suggests that HTLV-II Rex may increase expression of unspliced gag-pol mRNA, in part, by inhibiting splicing.

AB - The human T-cell leukemia virus type II (HTLV-II) regulatory protein Rex augments cytoplasmic levels of unspliced gag-pol mRNA by acting through a Rex-responsive element (RxRE) in the long terminal repeat. Purified Rex protein binds to long terminal repeat mRNA. Here, using an immunobinding assay to measure the binding of Rex protein to mutated RxRE RNAs, we show that efficient Rex binding requires a stem-bulge-loop RNA secondary structure (nucleotides [nt] 465 to 500) and specific sequences both within the stem-bulge (nt 470 to 476) and within a conserved upstream splice donor site (nt 449 to 455). Rex function in a transient transfection expression system correlates with Rex protein-RxRE RNA binding. The ability of HTLV-II Rex to interact directly with the HTLV-II splice donor site suggests that HTLV-II Rex may increase expression of unspliced gag-pol mRNA, in part, by inhibiting splicing.

UR - http://www.scopus.com/inward/record.url?scp=0025794389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025794389&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 6645

EP - 6653

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 12

ER -