Human transcription factor USF stimulates transcription through the initiator elements of the HIV-1 and the Ad-ML promoters

Hong Du, A. L. Roy, R. G. Roeder

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Earlier in vitro studies identified USF as a cellular factor which activates the adenovirus major late (Ad-ML) promoter by binding to an E-box motif located at position -60 with respect to the cap site. Purified USF contains 44 and 43 kDa polypeptides, and the latter was found (by cDNA cloning) to be a helix-loop-helix protein. In this report, we demonstrate a 25- to 30-fold stimulation of transcription via an upstream binding site by ectopic expression of the 43 kDa form of USF (USF 43 ) in transient transfection assays. More recent data have also revealed alternate interactions of USF 43 at pyrimidine-rich (consensus YYAYTCY) initiator (Inr) elements present in a variety of core promoters. In agreement with this observation, we show here that USF 43 can recognize the initiator elements of the HrV-1 promoter, as well as those in the Ad-ML promoter, and that ectopic expression of USF 43 can stimulate markedly the corresponding core promoters (TATA and initiator elements) when analyzed in transient co-transfection assays. Mutations in either Inr 1 or Inr 2 reduced the USF43-dependent transcription activity in vivo. In addition, in vitro transcription assays showed that mutations in either or both of the Inr 1 and Inr 2 sequences of the HIV-1 and Ad-ML promoters could affect transcription efficiency, but not the position of the transcriptional start site. These results indicate that USF 43 can stimulate transcription through initiator elements in two viral promoters, although the exact mechanism and physiological significance of this effect remain unclear.

Original languageEnglish (US)
Pages (from-to)501-511
Number of pages11
JournalEMBO Journal
Volume12
Issue number2
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Upstream Stimulatory Factors
Transcription
Adenoviridae
HIV-1
Transfection
Assays
E-Box Elements
Mutation
Organism Cloning
Complementary DNA
Binding Sites
Cloning
Peptides
Proteins
In Vitro Techniques
Ectopic Gene Expression

Keywords

  • Ad-ML and HIV-1 promoter
  • Initiator element
  • Transcription factor
  • USF

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Human transcription factor USF stimulates transcription through the initiator elements of the HIV-1 and the Ad-ML promoters. / Du, Hong; Roy, A. L.; Roeder, R. G.

In: EMBO Journal, Vol. 12, No. 2, 01.01.1993, p. 501-511.

Research output: Contribution to journalArticle

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