Human umbilical cord blood: A clinically useful source of transplantable hematopoietic stem/progenitor cells

Hal E. Broxmeyer, Scott Cooper, Giao Hangoc, Eliane Gluckman, Arleen Auerbach, Gordon W. Douglas, Henry Friedman, Joanne Kurtzberg, Judith Bard, Edward A. Boyse

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

This is a review and discussion of studies leading to the first use of human umbilical cord blood, material usually discarded, for the provision of stem/progenitor cells for clinical hematopoietic reconstitution [1,2]. This prospect arose as a result of extensive studies of the harvesting and cryopreservation of cord blood and of its numerical content of progenitor cells demonstrable in vitro. A male patient with Fanconi anemia (FA) was conditioned with a modified regimen of cyclophosphamide and irradiation that accommodates the abnormally high sensitivity to these agents that is characteristic of FA. Cryopreserved cord blood had been retrieved at birth from a female sibling known from prenatal testing to be unaffected by FA and to be human leukocyte antigen (HLA)‐compatible with the prospective sibling recipient. After conditioning and therapeutic infusion of thawed cord blood, successful hematopoietic reconstitution was indicated by the general health of the patient, who had previously required supportive transfusions, by satisfactory hematological criteria and by counts of hematopoietic progenitor cells of various types in the bone marrow. Complete engraftment of the myeloid system with donor cells was evident from cytogenetics, ABO typing, study of DNA polymorphisms, and normal cellular resistance to cytotoxic agents that reveal the fragility of FA cells; the blood contained a residuum of host lymphocytes exhibiting chromosomal damage, but the trend has been towards eliminating these damaged cells. This implies that cord blood from a single individual should provide sufficient reconstituting cells for effective hematopoietic repopulation of an autologous or an HLA‐compatible allogeneic recipient.

Original languageEnglish (US)
Pages (from-to)76-91
Number of pages16
JournalThe International Journal of Cell Cloning
Volume8
Issue number1 S
DOIs
StatePublished - 1990

Fingerprint

Fanconi Anemia
Hematopoietic Stem Cell Transplantation
HLA Antigens
Hematopoietic Stem Cells
Fetal Blood
Blood Transfusion
Stem Cells
Siblings
DNA Fingerprinting
Cryopreservation
Cytotoxins
Cytogenetics
Cyclophosphamide
Bone Marrow
Tissue Donors
Parturition
Lymphocytes
Health

Keywords

  • Hematopoietic stem/progenitor cells Hematopoietic reconstituting cells
  • Umbilical cord blood

ASJC Scopus subject areas

  • Cell Biology

Cite this

Human umbilical cord blood : A clinically useful source of transplantable hematopoietic stem/progenitor cells. / Broxmeyer, Hal E.; Cooper, Scott; Hangoc, Giao; Gluckman, Eliane; Auerbach, Arleen; Douglas, Gordon W.; Friedman, Henry; Kurtzberg, Joanne; Bard, Judith; Boyse, Edward A.

In: The International Journal of Cell Cloning, Vol. 8, No. 1 S, 1990, p. 76-91.

Research output: Contribution to journalArticle

Broxmeyer, HE, Cooper, S, Hangoc, G, Gluckman, E, Auerbach, A, Douglas, GW, Friedman, H, Kurtzberg, J, Bard, J & Boyse, EA 1990, 'Human umbilical cord blood: A clinically useful source of transplantable hematopoietic stem/progenitor cells', The International Journal of Cell Cloning, vol. 8, no. 1 S, pp. 76-91. https://doi.org/10.1002/stem.5530080708
Broxmeyer, Hal E. ; Cooper, Scott ; Hangoc, Giao ; Gluckman, Eliane ; Auerbach, Arleen ; Douglas, Gordon W. ; Friedman, Henry ; Kurtzberg, Joanne ; Bard, Judith ; Boyse, Edward A. / Human umbilical cord blood : A clinically useful source of transplantable hematopoietic stem/progenitor cells. In: The International Journal of Cell Cloning. 1990 ; Vol. 8, No. 1 S. pp. 76-91.
@article{deca9041191643d0825443c7ff4704f5,
title = "Human umbilical cord blood: A clinically useful source of transplantable hematopoietic stem/progenitor cells",
abstract = "This is a review and discussion of studies leading to the first use of human umbilical cord blood, material usually discarded, for the provision of stem/progenitor cells for clinical hematopoietic reconstitution [1,2]. This prospect arose as a result of extensive studies of the harvesting and cryopreservation of cord blood and of its numerical content of progenitor cells demonstrable in vitro. A male patient with Fanconi anemia (FA) was conditioned with a modified regimen of cyclophosphamide and irradiation that accommodates the abnormally high sensitivity to these agents that is characteristic of FA. Cryopreserved cord blood had been retrieved at birth from a female sibling known from prenatal testing to be unaffected by FA and to be human leukocyte antigen (HLA)‐compatible with the prospective sibling recipient. After conditioning and therapeutic infusion of thawed cord blood, successful hematopoietic reconstitution was indicated by the general health of the patient, who had previously required supportive transfusions, by satisfactory hematological criteria and by counts of hematopoietic progenitor cells of various types in the bone marrow. Complete engraftment of the myeloid system with donor cells was evident from cytogenetics, ABO typing, study of DNA polymorphisms, and normal cellular resistance to cytotoxic agents that reveal the fragility of FA cells; the blood contained a residuum of host lymphocytes exhibiting chromosomal damage, but the trend has been towards eliminating these damaged cells. This implies that cord blood from a single individual should provide sufficient reconstituting cells for effective hematopoietic repopulation of an autologous or an HLA‐compatible allogeneic recipient.",
keywords = "Hematopoietic stem/progenitor cells Hematopoietic reconstituting cells, Umbilical cord blood",
author = "Broxmeyer, {Hal E.} and Scott Cooper and Giao Hangoc and Eliane Gluckman and Arleen Auerbach and Douglas, {Gordon W.} and Henry Friedman and Joanne Kurtzberg and Judith Bard and Boyse, {Edward A.}",
year = "1990",
doi = "10.1002/stem.5530080708",
language = "English (US)",
volume = "8",
pages = "76--91",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "1 S",

}

TY - JOUR

T1 - Human umbilical cord blood

T2 - A clinically useful source of transplantable hematopoietic stem/progenitor cells

AU - Broxmeyer, Hal E.

AU - Cooper, Scott

AU - Hangoc, Giao

AU - Gluckman, Eliane

AU - Auerbach, Arleen

AU - Douglas, Gordon W.

AU - Friedman, Henry

AU - Kurtzberg, Joanne

AU - Bard, Judith

AU - Boyse, Edward A.

PY - 1990

Y1 - 1990

N2 - This is a review and discussion of studies leading to the first use of human umbilical cord blood, material usually discarded, for the provision of stem/progenitor cells for clinical hematopoietic reconstitution [1,2]. This prospect arose as a result of extensive studies of the harvesting and cryopreservation of cord blood and of its numerical content of progenitor cells demonstrable in vitro. A male patient with Fanconi anemia (FA) was conditioned with a modified regimen of cyclophosphamide and irradiation that accommodates the abnormally high sensitivity to these agents that is characteristic of FA. Cryopreserved cord blood had been retrieved at birth from a female sibling known from prenatal testing to be unaffected by FA and to be human leukocyte antigen (HLA)‐compatible with the prospective sibling recipient. After conditioning and therapeutic infusion of thawed cord blood, successful hematopoietic reconstitution was indicated by the general health of the patient, who had previously required supportive transfusions, by satisfactory hematological criteria and by counts of hematopoietic progenitor cells of various types in the bone marrow. Complete engraftment of the myeloid system with donor cells was evident from cytogenetics, ABO typing, study of DNA polymorphisms, and normal cellular resistance to cytotoxic agents that reveal the fragility of FA cells; the blood contained a residuum of host lymphocytes exhibiting chromosomal damage, but the trend has been towards eliminating these damaged cells. This implies that cord blood from a single individual should provide sufficient reconstituting cells for effective hematopoietic repopulation of an autologous or an HLA‐compatible allogeneic recipient.

AB - This is a review and discussion of studies leading to the first use of human umbilical cord blood, material usually discarded, for the provision of stem/progenitor cells for clinical hematopoietic reconstitution [1,2]. This prospect arose as a result of extensive studies of the harvesting and cryopreservation of cord blood and of its numerical content of progenitor cells demonstrable in vitro. A male patient with Fanconi anemia (FA) was conditioned with a modified regimen of cyclophosphamide and irradiation that accommodates the abnormally high sensitivity to these agents that is characteristic of FA. Cryopreserved cord blood had been retrieved at birth from a female sibling known from prenatal testing to be unaffected by FA and to be human leukocyte antigen (HLA)‐compatible with the prospective sibling recipient. After conditioning and therapeutic infusion of thawed cord blood, successful hematopoietic reconstitution was indicated by the general health of the patient, who had previously required supportive transfusions, by satisfactory hematological criteria and by counts of hematopoietic progenitor cells of various types in the bone marrow. Complete engraftment of the myeloid system with donor cells was evident from cytogenetics, ABO typing, study of DNA polymorphisms, and normal cellular resistance to cytotoxic agents that reveal the fragility of FA cells; the blood contained a residuum of host lymphocytes exhibiting chromosomal damage, but the trend has been towards eliminating these damaged cells. This implies that cord blood from a single individual should provide sufficient reconstituting cells for effective hematopoietic repopulation of an autologous or an HLA‐compatible allogeneic recipient.

KW - Hematopoietic stem/progenitor cells Hematopoietic reconstituting cells

KW - Umbilical cord blood

UR - http://www.scopus.com/inward/record.url?scp=0025031787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025031787&partnerID=8YFLogxK

U2 - 10.1002/stem.5530080708

DO - 10.1002/stem.5530080708

M3 - Article

C2 - 1969886

AN - SCOPUS:0025031787

VL - 8

SP - 76

EP - 91

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 1 S

ER -