Human xeroderma pigmentosum group A protein interacts with human replication protein A and inhibits DNA replication

Suk-Hee Lee, D. K. Kim, R. Drissi

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Human replication protein A (RPA; also known as human single-stranded DNA binding protein, or HSSB) is a multisubunit complex involved in both DNA replication and repair. While the role of RPA in replication has been well studied, its function in repair is less clear, although it is known to be involved in the early stages of the repair process. We found that RPA interacts with xeroderma pigmentosum group A complementing protein (XPAC), a protein that specifically recognizes UV-damaged DNA. We examined the effect of this XPAC-RPA interaction on in vitro simian virus 40 (SV40) DNA replication catalyzed by the monopolymerase system. XPAC inhibited SV40 DNA replication in vitro, and this inhibition was reversed by the addition of RPA but not by the addition of DNA polymerase α-primase complex, SV40 large tumor antigen, or topoisomerase I. This inhibition did not result from an interaction between XPAC and single-stranded DNA (ssDNA), or from competition between RPA and XPAC for DNA binding, because XPAC does not show any ssDNA binding activity and, in fact, stimulates RPA's ssDNA binding activity. Furthermore, XPAC inhibited DNA polymerase α activity in the presence of RPA but not in RPA's absence. These results suggest that the inhibitory effect of XPAC on DNA replication probably occurs through its interaction with RPA.

Original languageEnglish (US)
Pages (from-to)21800-21805
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number37
DOIs
StatePublished - 1995
Externally publishedYes

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Xeroderma Pigmentosum Group A Protein
DNA Replication
DNA
Simian virus 40
Single-Stranded DNA
Viruses
Repair
DNA-Directed DNA Polymerase
DNA Primase
Type I DNA Topoisomerase
human RPA1 protein
DNA-Binding Proteins
Neoplasm Antigens
Protein Binding
DNA Repair

ASJC Scopus subject areas

  • Biochemistry

Cite this

Human xeroderma pigmentosum group A protein interacts with human replication protein A and inhibits DNA replication. / Lee, Suk-Hee; Kim, D. K.; Drissi, R.

In: Journal of Biological Chemistry, Vol. 270, No. 37, 1995, p. 21800-21805.

Research output: Contribution to journalArticle

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