Hydrogen peroxide: A feed-forward dilator that couples myocardial metabolism to coronary blood flow

Shu Ichi Saitoh, Cuihua Zhang, Johnathan Tune, Barry Potter, Takahiko Kiyooka, Paul A. Rogers, Jarrod D. Knudson, Gregory M. Dick, Albert Swafford, William M. Chilian

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

OBJECTIVE - We tested the hypothesis that hydrogen peroxide (H2O2), the dismutated product of superoxide (O2), couples myocardial oxygen consumption to coronary blood flow. Accordingly, we measured O2 and H2O2 production by isolated cardiac myocytes, determined the role of mitochondrial electron transport in the production of these species, and determined the vasoactive properties of the produced H2O2. METHODS AND RESULTS - The production of O2 is coupled to oxidative metabolism because inhibition of complex I (rotenone) or III (antimycin) enhanced the production of O2 during pacing by about 50% and 400%, respectively; whereas uncoupling oxidative phosphorylation by decreasing the protonmotive force with carbonylcyanide-p-trifluoromethoxyphenyl-hydrazone (FCCP) decreased pacing-induced O2 production. The inhibitor of cytosolic NAD(P)H oxidase assembly, apocynin, did not affect O2 production by pacing. Aliquots of buffer from paced myocytes produced vasodilation of isolated arterioles (peak response 67±8% percent of maximal dilation) that was significantly reduced by catalase (5±0.5%, P

Original languageEnglish (US)
Pages (from-to)2614-2621
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume26
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Rotenone
Hydrazones
NADPH Oxidase
Oxidative Phosphorylation
Arterioles
Electron Transport
Cardiac Myocytes
Vasodilation
Oxygen Consumption
Superoxides
Catalase
Muscle Cells
Hydrogen Peroxide
Dilatation
Buffers
antimycin
acetovanillone

Keywords

  • Coronary circulation
  • Microcirculation
  • Reactive oxygen species
  • Vasodilation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Hydrogen peroxide : A feed-forward dilator that couples myocardial metabolism to coronary blood flow. / Saitoh, Shu Ichi; Zhang, Cuihua; Tune, Johnathan; Potter, Barry; Kiyooka, Takahiko; Rogers, Paul A.; Knudson, Jarrod D.; Dick, Gregory M.; Swafford, Albert; Chilian, William M.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 26, No. 12, 12.2006, p. 2614-2621.

Research output: Contribution to journalArticle

Saitoh, SI, Zhang, C, Tune, J, Potter, B, Kiyooka, T, Rogers, PA, Knudson, JD, Dick, GM, Swafford, A & Chilian, WM 2006, 'Hydrogen peroxide: A feed-forward dilator that couples myocardial metabolism to coronary blood flow', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 26, no. 12, pp. 2614-2621. https://doi.org/10.1161/01.ATV.0000249408.55796.da
Saitoh, Shu Ichi ; Zhang, Cuihua ; Tune, Johnathan ; Potter, Barry ; Kiyooka, Takahiko ; Rogers, Paul A. ; Knudson, Jarrod D. ; Dick, Gregory M. ; Swafford, Albert ; Chilian, William M. / Hydrogen peroxide : A feed-forward dilator that couples myocardial metabolism to coronary blood flow. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2006 ; Vol. 26, No. 12. pp. 2614-2621.
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