Hyperbaric oxygen improves engraftment of ex-vivo expanded and gene transduced human CD34+ cells in a murine model of umbilical cord blood transplantation

Omar S. Aljitawi, Yinghua Xiao, Jeff D. Eskew, Nikhil K. Parelkar, Megan Swink, Jeff Radel, Tara L. Lin, Bruce F. Kimler, Jonathan D. Mahnken, Joseph P. McGuirk, Hal Broxmeyer, George Vielhauer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Delayed engraftment and graft failure represent major obstacles to successful umbilical cord blood (UCB) transplantation. Herein, we evaluated the use of hyperbaric oxygen (HBO) therapy as an intervention to improve human UCB stem/progenitor cell engraftment in an immune deficient mouse model. Six- to eight-week old NSG mice were sublethally irradiated 24hours prior to CD34+ UCB cell transplant. Irradiated mice were separated into a non-HBO group (where mice remained under normoxic conditions) and the HBO group (where mice received 2 hours of HBO therapy; 100% oxygen at 2.5 atmospheres absolute). Four hours after completing HBO therapy, both groups intravenously received CD34+ UCB cells that were transduced with a lentivirus carrying luciferase gene and expanded for in vivo imaging. Mice were imaged and then sacrificed at one of 10 times up to 4.5months post-transplant. HBO treated mice demonstrated significantly improved bone marrow, peripheral blood, and spleen retention and subsequent engraftment. In addition, HBO significantly improved peripheral, spleen and bone marrow engraftment of human myeloid and B-cell subsets. In vivo imaging demonstrated that HBO mice had significantly higher ventral and dorsal bioluminescence values. These studies suggest that HBO treatment of NSG mice prior to UCB CD34+ cell infusion significantly improves engraftment.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalBlood Cells, Molecules and Diseases
Volume52
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Fetal Blood
Transplantation
Oxygen
Genes
Hyperbaric Oxygenation
Blood Cells
Transplants
Stem Cells
Spleen
Bone Marrow
B-Lymphocyte Subsets
Lentivirus
Myeloid Cells
Luciferases
Atmosphere

Keywords

  • CD34
  • Engraftment
  • Hyperbaric oxygen
  • Umbilical cord blood

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology

Cite this

Hyperbaric oxygen improves engraftment of ex-vivo expanded and gene transduced human CD34+ cells in a murine model of umbilical cord blood transplantation. / Aljitawi, Omar S.; Xiao, Yinghua; Eskew, Jeff D.; Parelkar, Nikhil K.; Swink, Megan; Radel, Jeff; Lin, Tara L.; Kimler, Bruce F.; Mahnken, Jonathan D.; McGuirk, Joseph P.; Broxmeyer, Hal; Vielhauer, George.

In: Blood Cells, Molecules and Diseases, Vol. 52, No. 1, 01.2014, p. 59-67.

Research output: Contribution to journalArticle

Aljitawi, OS, Xiao, Y, Eskew, JD, Parelkar, NK, Swink, M, Radel, J, Lin, TL, Kimler, BF, Mahnken, JD, McGuirk, JP, Broxmeyer, H & Vielhauer, G 2014, 'Hyperbaric oxygen improves engraftment of ex-vivo expanded and gene transduced human CD34+ cells in a murine model of umbilical cord blood transplantation', Blood Cells, Molecules and Diseases, vol. 52, no. 1, pp. 59-67. https://doi.org/10.1016/j.bcmd.2013.07.013
Aljitawi, Omar S. ; Xiao, Yinghua ; Eskew, Jeff D. ; Parelkar, Nikhil K. ; Swink, Megan ; Radel, Jeff ; Lin, Tara L. ; Kimler, Bruce F. ; Mahnken, Jonathan D. ; McGuirk, Joseph P. ; Broxmeyer, Hal ; Vielhauer, George. / Hyperbaric oxygen improves engraftment of ex-vivo expanded and gene transduced human CD34+ cells in a murine model of umbilical cord blood transplantation. In: Blood Cells, Molecules and Diseases. 2014 ; Vol. 52, No. 1. pp. 59-67.
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