Hyperinsulinemic-euglycemic clamps in conscious, unrestrained mice.

Julio E. Ayala, Deanna P. Bracy, Carlo Malabanan, Freyja D. James, Tasneem Ansari, Patrick T. Fueger, Owen P. McGuinness, David H. Wasserman

Research output: Contribution to journalArticle

Abstract

Type 2 diabetes is characterized by a defect in insulin action. The hyperinsulinemic-euglycemic clamp, or insulin clamp, is widely considered the "gold standard" method for assessing insulin action in vivo. During an insulin clamp, hyperinsulinemia is achieved by a constant insulin infusion. Euglycemia is maintained via a concomitant glucose infusion at a variable rate. This variable glucose infusion rate (GIR) is determined by measuring blood glucose at brief intervals throughout the experiment and adjusting the GIR accordingly. The GIR is indicative of whole-body insulin action, as mice with enhanced insulin action require a greater GIR. The insulin clamp can incorporate administration of isotopic 2[(14)C]deoxyglucose to assess tissue-specific glucose uptake and [3-(3)H]glucose to assess the ability of insulin to suppress the rate of endogenous glucose appearance (endoRa), a marker of hepatic glucose production, and to stimulate the rate of whole-body glucose disappearance (Rd). The miniaturization of the insulin clamp for use in genetic mouse models of metabolic disease has led to significant advances in diabetes research. Methods for performing insulin clamps vary between laboratories. It is important to note that the manner in which an insulin clamp is performed can significantly affect the results obtained. We have published a comprehensive assessment of different approaches to performing insulin clamps in conscious mice(1) as well as an evaluation of the metabolic response of four commonly used inbred mouse strains using various clamp techniques(2). Here we present a protocol for performing insulin clamps on conscious, unrestrained mice developed by the Vanderbilt Mouse Metabolic Phenotyping Center (MMPC; URL: www.mc.vanderbilt.edu/mmpc). This includes a description of the method for implanting catheters used during the insulin clamp. The protocol employed by the Vanderbilt MMPC utilizes a unique two-catheter system(3). One catheter is inserted into the jugular vein for infusions. A second catheter is inserted into the carotid artery, which allows for blood sampling without the need to restrain or handle the mouse. This technique provides a significant advantage to the most common method for obtaining blood samples during insulin clamps which is to sample from the severed tip of the tail. Unlike this latter method, sampling from an arterial catheter is not stressful to the mouse(1). We also describe methods for using isotopic tracer infusions to assess tissue-specific insulin action. We also provide guidelines for the appropriate presentation of results obtained from insulin clamps.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number57
StatePublished - 2011
Externally publishedYes

Fingerprint

Glucose Clamp Technique
Insulin
Clamping devices
Glucose
Catheters
Blood
Medical problems
Tissue
Sampling
Miniaturization
Inbred Strains Mice
Genetic Models
Metabolic Diseases
Jugular Veins
Deoxyglucose
Hyperinsulinism

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ayala, J. E., Bracy, D. P., Malabanan, C., James, F. D., Ansari, T., Fueger, P. T., ... Wasserman, D. H. (2011). Hyperinsulinemic-euglycemic clamps in conscious, unrestrained mice. Journal of visualized experiments : JoVE, (57).

Hyperinsulinemic-euglycemic clamps in conscious, unrestrained mice. / Ayala, Julio E.; Bracy, Deanna P.; Malabanan, Carlo; James, Freyja D.; Ansari, Tasneem; Fueger, Patrick T.; McGuinness, Owen P.; Wasserman, David H.

In: Journal of visualized experiments : JoVE, No. 57, 2011.

Research output: Contribution to journalArticle

Ayala, JE, Bracy, DP, Malabanan, C, James, FD, Ansari, T, Fueger, PT, McGuinness, OP & Wasserman, DH 2011, 'Hyperinsulinemic-euglycemic clamps in conscious, unrestrained mice.', Journal of visualized experiments : JoVE, no. 57.
Ayala JE, Bracy DP, Malabanan C, James FD, Ansari T, Fueger PT et al. Hyperinsulinemic-euglycemic clamps in conscious, unrestrained mice. Journal of visualized experiments : JoVE. 2011;(57).
Ayala, Julio E. ; Bracy, Deanna P. ; Malabanan, Carlo ; James, Freyja D. ; Ansari, Tasneem ; Fueger, Patrick T. ; McGuinness, Owen P. ; Wasserman, David H. / Hyperinsulinemic-euglycemic clamps in conscious, unrestrained mice. In: Journal of visualized experiments : JoVE. 2011 ; No. 57.
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