Hyperpolarization-activated chloride currents in Xenopus oocytes

Gopal C. Kowdley, Stephen J. Ackerman, J. Edward John, Larry R. Jones, J. Randall Moorman

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

During hyperpolarizing pulses, defolliculated Xenopus oocytes have time- and voltage-dependent inward chloride currents. The currents vary greatly in amplitude from batch to batch; activate slowly and, in general, do not decay; have a selectivity sequence of I- > NO3/- > Br- > Cl- > propionate > acetate; are insensitive to Ca2+ and pH; are blocked by Ba2+ and some chloride channel blockers; and have a gating valence of ~1.3 charges. In contrast to hyperpolarization-activated chloride currents induced after expression of phospholemman (Palmer, C. J., B. T. Scott, and L. R. Jones. 1991. Journal of Biological Chemistry. 266:11126; Moorman, J. R., C. J. Palmer, J. E. John, J. E. Durieux, and L. R. Jones. 1992. 267:14551), these endogenous currents are smaller; have a different pharmacologic profile; have a lower threshold for activation and lower voltage-sensitivity of activation; have different activation kinetics; and are insensitive to pH. Nonetheless, the endogenous and expressed current share striking similarities. Recordings of macroscopic oocyte currents may be inadequate to determine whether phospholemman is itself an ion channel and not a channel-modulating molecule.

Original languageEnglish (US)
Pages (from-to)217-230
Number of pages14
JournalJournal of General Physiology
Volume103
Issue number2
DOIs
StatePublished - Feb 1994

ASJC Scopus subject areas

  • Physiology

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