Hypertonie activation and recovery of system a amino acid transport in renal MDCK cells

Jie Guang Chen, Mark Coe, James A. McAteer, Stephen A. Kempson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Amino compounds are abundant within the renal inner medulla, but their possible role during hypertonic stress is not clear. Renal epithelial Madin-Darby canine kidney cells were used to examine the osmoregulation of system A transport, a major Na+-dependent process for neutral amino acid transport. System A activity was markedly increased after 6 h of hypertonic challenge, and intracellular alanine content increased more than twofold. The activation of system A was reversed after 24 h of hypertonic challenge. This downregulation was accompanied by the activation of betaine transport, as measured by -j'-aminobutyric acid uptake. Extracellular betaine prevented the early activation of system A. The hypertonic activation of system A was blocked by actinomycin D and cycloheximide. When cells were returned to isotonic medium after hypertonic activation, the recovery of system A transport also was partially inhibited by actinomycin D and puromycin. The results are consistent with the possibility that hypertonicity, by disrupting a represser protein, leads to increased synthesis of a system A-related protein. The isotonic recovery may require synthesis of new repressor proteins.

Original languageEnglish
JournalThe American journal of physiology
Volume270
Issue number3 PART 2
StatePublished - 1996

Fingerprint

Amino Acid Transport Systems
Betaine
Madin Darby Canine Kidney Cells
Dactinomycin
Repressor Proteins
Aminobutyrates
Neutral Amino Acids
Kidney
Osmoregulation
Puromycin
Osmotic Pressure
Cycloheximide
Alanine
Proteins
Down-Regulation
Epithelial Cells

Keywords

  • Alanine
  • Betaine
  • Carbon-14-labeled u-methylaminoisobutyric acid
  • Kidney
  • Madin-darby canine kidney cells
  • Osmoregulation

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Chen, J. G., Coe, M., McAteer, J. A., & Kempson, S. A. (1996). Hypertonie activation and recovery of system a amino acid transport in renal MDCK cells. The American journal of physiology, 270(3 PART 2).

Hypertonie activation and recovery of system a amino acid transport in renal MDCK cells. / Chen, Jie Guang; Coe, Mark; McAteer, James A.; Kempson, Stephen A.

In: The American journal of physiology, Vol. 270, No. 3 PART 2, 1996.

Research output: Contribution to journalArticle

Chen, Jie Guang ; Coe, Mark ; McAteer, James A. ; Kempson, Stephen A. / Hypertonie activation and recovery of system a amino acid transport in renal MDCK cells. In: The American journal of physiology. 1996 ; Vol. 270, No. 3 PART 2.
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