Hypoxia induces different genes in the lungs of rats compared with mice

Yasushi Hoshikawa, Patrick Nana-Sinkam, Mark D. Moore, Sylk Sotto-Santiago, Tzulip Phang, Robert L. Keith, Kenneth G. Morris, Takashi Kondo, Rubin M. Tuder, Norbert F. Voelkel, Mark W. Geraci

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Different animal species have a varying response to hypoxia. Mice develop less pulmonary artery thickening after chronic hypoxia exposure than rats. We hypothesized that the lung tissue gene expression pattern displayed in hypoxic rats would differ from that of hypoxic mice. We exposed Sprague-Dawley rats and C57BL/6 mice to both 1 and 3 wk of hypobaric hypoxia. Although both species developed pulmonary hypertension, mice showed less pulmonary vascular remodeling than rats. Microarray gene analysis demonstrated a distinct pattern of gene expression between mice and rats when exposed to hypoxic conditions. In addition, some genes appeared to be more responsive at an earlier time point of 1 wk of hypoxia. Hypoxic conditions in the rat induce genes involved in endothelial cell proliferation, repression of apoptosis, and vasodilation. Mice exposed to hypoxic conditions decrease the expression of genes involved in vasodilation and in endothelial cell proliferation. Although we cannot determine whether the differential expression of genes during chronic hypoxia is cause or consequence of the differential pulmonary vascular remodeling, we propose that a balance between over- and under-expression of a selective group of genes may be responsible for lung vascular remodeling and vascular tone control.

Original languageEnglish (US)
Pages (from-to)209-219
Number of pages11
JournalPhysiological Genomics
Volume12
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

Lung
Gene Expression
Genes
Vasodilation
Endothelial Cells
Cell Proliferation
Microarray Analysis
Inbred C57BL Mouse
Pulmonary Hypertension
Pulmonary Artery
Blood Vessels
Sprague Dawley Rats
Hypoxia
Apoptosis
Vascular Remodeling

Keywords

  • Chronic hypoxia
  • Gene microarray analysis
  • Pulmonary hypertension
  • Pulmonary vascular remodeling
  • Species differences

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Hoshikawa, Y., Nana-Sinkam, P., Moore, M. D., Sotto-Santiago, S., Phang, T., Keith, R. L., ... Geraci, M. W. (2003). Hypoxia induces different genes in the lungs of rats compared with mice. Physiological Genomics, 12, 209-219.

Hypoxia induces different genes in the lungs of rats compared with mice. / Hoshikawa, Yasushi; Nana-Sinkam, Patrick; Moore, Mark D.; Sotto-Santiago, Sylk; Phang, Tzulip; Keith, Robert L.; Morris, Kenneth G.; Kondo, Takashi; Tuder, Rubin M.; Voelkel, Norbert F.; Geraci, Mark W.

In: Physiological Genomics, Vol. 12, 04.2003, p. 209-219.

Research output: Contribution to journalArticle

Hoshikawa, Y, Nana-Sinkam, P, Moore, MD, Sotto-Santiago, S, Phang, T, Keith, RL, Morris, KG, Kondo, T, Tuder, RM, Voelkel, NF & Geraci, MW 2003, 'Hypoxia induces different genes in the lungs of rats compared with mice', Physiological Genomics, vol. 12, pp. 209-219.
Hoshikawa Y, Nana-Sinkam P, Moore MD, Sotto-Santiago S, Phang T, Keith RL et al. Hypoxia induces different genes in the lungs of rats compared with mice. Physiological Genomics. 2003 Apr;12:209-219.
Hoshikawa, Yasushi ; Nana-Sinkam, Patrick ; Moore, Mark D. ; Sotto-Santiago, Sylk ; Phang, Tzulip ; Keith, Robert L. ; Morris, Kenneth G. ; Kondo, Takashi ; Tuder, Rubin M. ; Voelkel, Norbert F. ; Geraci, Mark W. / Hypoxia induces different genes in the lungs of rats compared with mice. In: Physiological Genomics. 2003 ; Vol. 12. pp. 209-219.
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