Hypoxia induces HSP 70 gene expression in human hepatoma (HEP G2) cells

B. Patel, A. Khaliq, J. Jarvis-Evans, M. Boulton, S. Arrol, M. Mackness, D. McLeod

Research output: Contribution to journalArticle

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Abstract

Populations of cells within solid tumours are exposed to low oxygen concentrations. The mechanism by which tumour cells tolerate such hypoxia is unknown but it may parallel responses to other types of cellular stress. We investigated the effect of oxygen on steady state levels of inducible heat shock protein 70 mRNA in cultured human hepatoma cells. Northern blot analysis demonstrated that hypoxia increased HSP 70 mRNA levels within 3 hours, with a transient 12-fold increase at 6 hours compared with normoxia. We also showed that heat shock induced a 20-fold increase in HSP 70 mRNA. This data suggests that HSPs may be important in tumour progression by protecting cells from hypoxic stress.

Original languageEnglish (US)
Pages (from-to)907-912
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume36
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Gene expression
Tumors
Hepatocellular Carcinoma
Gene Expression
Messenger RNA
Cells
Oxygen
HSP70 Heat-Shock Proteins
Neoplasms
Northern Blotting
Shock
Hot Temperature
Hypoxia
Population

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Patel, B., Khaliq, A., Jarvis-Evans, J., Boulton, M., Arrol, S., Mackness, M., & McLeod, D. (1995). Hypoxia induces HSP 70 gene expression in human hepatoma (HEP G2) cells. Biochemistry and Molecular Biology International, 36(4), 907-912.

Hypoxia induces HSP 70 gene expression in human hepatoma (HEP G2) cells. / Patel, B.; Khaliq, A.; Jarvis-Evans, J.; Boulton, M.; Arrol, S.; Mackness, M.; McLeod, D.

In: Biochemistry and Molecular Biology International, Vol. 36, No. 4, 1995, p. 907-912.

Research output: Contribution to journalArticle

Patel, B, Khaliq, A, Jarvis-Evans, J, Boulton, M, Arrol, S, Mackness, M & McLeod, D 1995, 'Hypoxia induces HSP 70 gene expression in human hepatoma (HEP G2) cells', Biochemistry and Molecular Biology International, vol. 36, no. 4, pp. 907-912.
Patel B, Khaliq A, Jarvis-Evans J, Boulton M, Arrol S, Mackness M et al. Hypoxia induces HSP 70 gene expression in human hepatoma (HEP G2) cells. Biochemistry and Molecular Biology International. 1995;36(4):907-912.
Patel, B. ; Khaliq, A. ; Jarvis-Evans, J. ; Boulton, M. ; Arrol, S. ; Mackness, M. ; McLeod, D. / Hypoxia induces HSP 70 gene expression in human hepatoma (HEP G2) cells. In: Biochemistry and Molecular Biology International. 1995 ; Vol. 36, No. 4. pp. 907-912.
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