Hypoxia-inducible factor-1α regulates CD55 in airway epithelium

Pankita H. Pandya, Amanda J. Fisher, Elizabeth A. Mickler, Constance J. Temm, Kelsey P. Lipking, Adam Gracon, Katia Rothhaar, George Sandusky, Mary Murray, Karen Pollok, Jamie Renbarger, Janice Blum, Tim Lahm, David S. Wilkes

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Airway epithelial CD55 down-regulation occurs in several hypoxiaassociated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxiainducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on smallairway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxiarecovered CD55 expression in small-airway epithelial cells.HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium.

Original languageEnglish (US)
Pages (from-to)889-898
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume55
Issue number6
DOIs
StatePublished - Dec 1 2016

Fingerprint

Hypoxia-Inducible Factor 1
Epithelium
Epithelial Cells
Complement Activation
Gene Silencing
Pulmonary diseases
Lung Diseases
Down-Regulation
Assays
Lung
Genes
Chemical activation
In Vitro Techniques

Keywords

  • Complement regulatory proteins
  • Hypoxia
  • Lung

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Pandya, P. H., Fisher, A. J., Mickler, E. A., Temm, C. J., Lipking, K. P., Gracon, A., ... Wilkes, D. S. (2016). Hypoxia-inducible factor-1α regulates CD55 in airway epithelium. American Journal of Respiratory Cell and Molecular Biology, 55(6), 889-898. https://doi.org/10.1165/rcmb.2015-0237OC

Hypoxia-inducible factor-1α regulates CD55 in airway epithelium. / Pandya, Pankita H.; Fisher, Amanda J.; Mickler, Elizabeth A.; Temm, Constance J.; Lipking, Kelsey P.; Gracon, Adam; Rothhaar, Katia; Sandusky, George; Murray, Mary; Pollok, Karen; Renbarger, Jamie; Blum, Janice; Lahm, Tim; Wilkes, David S.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 55, No. 6, 01.12.2016, p. 889-898.

Research output: Contribution to journalArticle

Pandya, PH, Fisher, AJ, Mickler, EA, Temm, CJ, Lipking, KP, Gracon, A, Rothhaar, K, Sandusky, G, Murray, M, Pollok, K, Renbarger, J, Blum, J, Lahm, T & Wilkes, DS 2016, 'Hypoxia-inducible factor-1α regulates CD55 in airway epithelium', American Journal of Respiratory Cell and Molecular Biology, vol. 55, no. 6, pp. 889-898. https://doi.org/10.1165/rcmb.2015-0237OC
Pandya, Pankita H. ; Fisher, Amanda J. ; Mickler, Elizabeth A. ; Temm, Constance J. ; Lipking, Kelsey P. ; Gracon, Adam ; Rothhaar, Katia ; Sandusky, George ; Murray, Mary ; Pollok, Karen ; Renbarger, Jamie ; Blum, Janice ; Lahm, Tim ; Wilkes, David S. / Hypoxia-inducible factor-1α regulates CD55 in airway epithelium. In: American Journal of Respiratory Cell and Molecular Biology. 2016 ; Vol. 55, No. 6. pp. 889-898.
@article{04b22da2fbfd4abb8671c20e1abe534c,
title = "Hypoxia-inducible factor-1α regulates CD55 in airway epithelium",
abstract = "Airway epithelial CD55 down-regulation occurs in several hypoxiaassociated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxiainducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on smallairway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxiarecovered CD55 expression in small-airway epithelial cells.HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium.",
keywords = "Complement regulatory proteins, Hypoxia, Lung",
author = "Pandya, {Pankita H.} and Fisher, {Amanda J.} and Mickler, {Elizabeth A.} and Temm, {Constance J.} and Lipking, {Kelsey P.} and Adam Gracon and Katia Rothhaar and George Sandusky and Mary Murray and Karen Pollok and Jamie Renbarger and Janice Blum and Tim Lahm and Wilkes, {David S.}",
year = "2016",
month = "12",
day = "1",
doi = "10.1165/rcmb.2015-0237OC",
language = "English (US)",
volume = "55",
pages = "889--898",
journal = "American Journal of Respiratory Cell and Molecular Biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "6",

}

TY - JOUR

T1 - Hypoxia-inducible factor-1α regulates CD55 in airway epithelium

AU - Pandya, Pankita H.

AU - Fisher, Amanda J.

AU - Mickler, Elizabeth A.

AU - Temm, Constance J.

AU - Lipking, Kelsey P.

AU - Gracon, Adam

AU - Rothhaar, Katia

AU - Sandusky, George

AU - Murray, Mary

AU - Pollok, Karen

AU - Renbarger, Jamie

AU - Blum, Janice

AU - Lahm, Tim

AU - Wilkes, David S.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Airway epithelial CD55 down-regulation occurs in several hypoxiaassociated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxiainducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on smallairway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxiarecovered CD55 expression in small-airway epithelial cells.HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium.

AB - Airway epithelial CD55 down-regulation occurs in several hypoxiaassociated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxiainducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on smallairway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxiarecovered CD55 expression in small-airway epithelial cells.HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium.

KW - Complement regulatory proteins

KW - Hypoxia

KW - Lung

UR - http://www.scopus.com/inward/record.url?scp=85002644516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85002644516&partnerID=8YFLogxK

U2 - 10.1165/rcmb.2015-0237OC

DO - 10.1165/rcmb.2015-0237OC

M3 - Article

C2 - 27494303

AN - SCOPUS:85002644516

VL - 55

SP - 889

EP - 898

JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

IS - 6

ER -