Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression

Karen Cowden Dahl, Sarah E. Robertson, Valerie M. Weaver, M. Celeste Simon

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Hypoxia-inducible factor (HIF)-deficient placentas exhibit a number of defects, including changes in cell fate adoption, lack of fetal angiogenesis, hypocellularity, and poor invasion into maternal tissue. HIF is a heterodimeric transcription factor consisting of α and β aryl hydrocarbon receptor nuclear translocator or ARNT) subunits. We used undifferentiated trophoblast stem (TS) cells to characterize HIF-dependent adhesion, migration, and invasion. Arnt-/- and Hifα-/- TS cells exhibit reduced adhesion and migration toward vitronectin compared with wild-type cells. Furthermore, this defect is associated with decreased cell surface expression of integrin αvβ3 and significantly decreased expression of this integrin in focal adhesions. Because of the importance of adhesion and migration in tumor progression (in addition to placental development), we examined the affect of culturing B16F0 melanoma cells in 1.5% oxygen (O2). Culturing B16F0 melanoma cells at 1.5% O2 resulted in increased αvβ3 integrin surface expression and increased adhesion to and migration toward vitronectin. Together, these data suggest that HIF and O 2 tension influence placental invasion and tumor migration by increasing cell surface expression of αvβ3 integrin.

Original languageEnglish (US)
Pages (from-to)1901-1912
Number of pages12
JournalMolecular Biology of the Cell
Volume16
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

Fingerprint

Integrins
Vitronectin
Trophoblasts
Melanoma
Stem Cells
Aryl Hydrocarbon Receptor Nuclear Translocator
Placentation
Focal Adhesions
Placenta
Cell Movement
Neoplasms
Transcription Factors
Mothers
Hypoxia
Oxygen

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cowden Dahl, K., Robertson, S. E., Weaver, V. M., & Simon, M. C. (2005). Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression. Molecular Biology of the Cell, 16(4), 1901-1912. https://doi.org/10.1091/mbc.E04-12-1082

Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression. / Cowden Dahl, Karen; Robertson, Sarah E.; Weaver, Valerie M.; Simon, M. Celeste.

In: Molecular Biology of the Cell, Vol. 16, No. 4, 04.2005, p. 1901-1912.

Research output: Contribution to journalArticle

Cowden Dahl, K, Robertson, SE, Weaver, VM & Simon, MC 2005, 'Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression', Molecular Biology of the Cell, vol. 16, no. 4, pp. 1901-1912. https://doi.org/10.1091/mbc.E04-12-1082
Cowden Dahl, Karen ; Robertson, Sarah E. ; Weaver, Valerie M. ; Simon, M. Celeste. / Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 4. pp. 1901-1912.
@article{0d6304a2c5084fedbbd98a50ead83d88,
title = "Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression",
abstract = "Hypoxia-inducible factor (HIF)-deficient placentas exhibit a number of defects, including changes in cell fate adoption, lack of fetal angiogenesis, hypocellularity, and poor invasion into maternal tissue. HIF is a heterodimeric transcription factor consisting of α and β aryl hydrocarbon receptor nuclear translocator or ARNT) subunits. We used undifferentiated trophoblast stem (TS) cells to characterize HIF-dependent adhesion, migration, and invasion. Arnt-/- and Hifα-/- TS cells exhibit reduced adhesion and migration toward vitronectin compared with wild-type cells. Furthermore, this defect is associated with decreased cell surface expression of integrin αvβ3 and significantly decreased expression of this integrin in focal adhesions. Because of the importance of adhesion and migration in tumor progression (in addition to placental development), we examined the affect of culturing B16F0 melanoma cells in 1.5{\%} oxygen (O2). Culturing B16F0 melanoma cells at 1.5{\%} O2 resulted in increased αvβ3 integrin surface expression and increased adhesion to and migration toward vitronectin. Together, these data suggest that HIF and O 2 tension influence placental invasion and tumor migration by increasing cell surface expression of αvβ3 integrin.",
author = "{Cowden Dahl}, Karen and Robertson, {Sarah E.} and Weaver, {Valerie M.} and Simon, {M. Celeste}",
year = "2005",
month = "4",
doi = "10.1091/mbc.E04-12-1082",
language = "English (US)",
volume = "16",
pages = "1901--1912",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "4",

}

TY - JOUR

T1 - Hypoxia-inducible factor regulates αvβ3 integrin cell surface expression

AU - Cowden Dahl, Karen

AU - Robertson, Sarah E.

AU - Weaver, Valerie M.

AU - Simon, M. Celeste

PY - 2005/4

Y1 - 2005/4

N2 - Hypoxia-inducible factor (HIF)-deficient placentas exhibit a number of defects, including changes in cell fate adoption, lack of fetal angiogenesis, hypocellularity, and poor invasion into maternal tissue. HIF is a heterodimeric transcription factor consisting of α and β aryl hydrocarbon receptor nuclear translocator or ARNT) subunits. We used undifferentiated trophoblast stem (TS) cells to characterize HIF-dependent adhesion, migration, and invasion. Arnt-/- and Hifα-/- TS cells exhibit reduced adhesion and migration toward vitronectin compared with wild-type cells. Furthermore, this defect is associated with decreased cell surface expression of integrin αvβ3 and significantly decreased expression of this integrin in focal adhesions. Because of the importance of adhesion and migration in tumor progression (in addition to placental development), we examined the affect of culturing B16F0 melanoma cells in 1.5% oxygen (O2). Culturing B16F0 melanoma cells at 1.5% O2 resulted in increased αvβ3 integrin surface expression and increased adhesion to and migration toward vitronectin. Together, these data suggest that HIF and O 2 tension influence placental invasion and tumor migration by increasing cell surface expression of αvβ3 integrin.

AB - Hypoxia-inducible factor (HIF)-deficient placentas exhibit a number of defects, including changes in cell fate adoption, lack of fetal angiogenesis, hypocellularity, and poor invasion into maternal tissue. HIF is a heterodimeric transcription factor consisting of α and β aryl hydrocarbon receptor nuclear translocator or ARNT) subunits. We used undifferentiated trophoblast stem (TS) cells to characterize HIF-dependent adhesion, migration, and invasion. Arnt-/- and Hifα-/- TS cells exhibit reduced adhesion and migration toward vitronectin compared with wild-type cells. Furthermore, this defect is associated with decreased cell surface expression of integrin αvβ3 and significantly decreased expression of this integrin in focal adhesions. Because of the importance of adhesion and migration in tumor progression (in addition to placental development), we examined the affect of culturing B16F0 melanoma cells in 1.5% oxygen (O2). Culturing B16F0 melanoma cells at 1.5% O2 resulted in increased αvβ3 integrin surface expression and increased adhesion to and migration toward vitronectin. Together, these data suggest that HIF and O 2 tension influence placental invasion and tumor migration by increasing cell surface expression of αvβ3 integrin.

UR - http://www.scopus.com/inward/record.url?scp=16344378769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16344378769&partnerID=8YFLogxK

U2 - 10.1091/mbc.E04-12-1082

DO - 10.1091/mbc.E04-12-1082

M3 - Article

VL - 16

SP - 1901

EP - 1912

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 4

ER -