Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1

Olga V. Volpert, Roberto Pili, Hashmat A. Sikder, Thomas Nelius, Tetiana Zaichuk, Chad Morris, Clinton B. Shiflett, Meghann K. Devlin, Katherine Conant, Rhoda M. Alani

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Id proteins are helix-loop-helix transcription factors that regulate tumor angiogenesis. In order to identify downstream effectors of Id1 involved in the regulation of angiogenesis, we performed PCR-select subtractive hybridization on wild-type and Id1 knockout mouse embryo fibroblasts (MEFs). Here we demonstrate that thrombospondin-1 (TSP-1), a potent inhibitor of angiogenesis, is a target of transcriptional repression by Id1. We also show that Id1-null MEFs secrete an inhibitor of endothelial cell migration, which is completely inactivated by depletion of TSP-1. Furthermore, in vivo studies revealed decreased neovascularization in matrigel assays in Id1-null mice compared to their wild-type littermates. This decrease was completely reversed by a TSP-1 neutralizing antibody. We conclude that TSP-1 is a major target for Id1 effects on angiogenesis.

Original languageEnglish (US)
Pages (from-to)473-483
Number of pages11
JournalCancer Cell
Volume2
Issue number6
DOIs
StatePublished - Dec 2002
Externally publishedYes

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Thrombospondin 1
Embryonic Structures
Fibroblasts
Angiogenesis Inhibitors
Neutralizing Antibodies
Knockout Mice
Cell Movement
Transcription Factors
Endothelial Cells
Polymerase Chain Reaction
Neoplasms
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

Cite this

Volpert, O. V., Pili, R., Sikder, H. A., Nelius, T., Zaichuk, T., Morris, C., ... Alani, R. M. (2002). Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1. Cancer Cell, 2(6), 473-483. https://doi.org/10.1016/S1535-6108(02)00209-X

Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1. / Volpert, Olga V.; Pili, Roberto; Sikder, Hashmat A.; Nelius, Thomas; Zaichuk, Tetiana; Morris, Chad; Shiflett, Clinton B.; Devlin, Meghann K.; Conant, Katherine; Alani, Rhoda M.

In: Cancer Cell, Vol. 2, No. 6, 12.2002, p. 473-483.

Research output: Contribution to journalArticle

Volpert, OV, Pili, R, Sikder, HA, Nelius, T, Zaichuk, T, Morris, C, Shiflett, CB, Devlin, MK, Conant, K & Alani, RM 2002, 'Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1', Cancer Cell, vol. 2, no. 6, pp. 473-483. https://doi.org/10.1016/S1535-6108(02)00209-X
Volpert, Olga V. ; Pili, Roberto ; Sikder, Hashmat A. ; Nelius, Thomas ; Zaichuk, Tetiana ; Morris, Chad ; Shiflett, Clinton B. ; Devlin, Meghann K. ; Conant, Katherine ; Alani, Rhoda M. / Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1. In: Cancer Cell. 2002 ; Vol. 2, No. 6. pp. 473-483.
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