Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor β

Keisuke Horiuchi, Norio Amizuka, Sunao Takeshita, Hiroyuki Takamatsu, Mieko Katsuura, Hidehiro Ozawa, Yoshiaki Toyama, Lynda Bonewald, Akira Kudo

Research output: Contribution to journalArticle

656 Citations (Scopus)

Abstract

We had previously identified the cDNA for a novel protein called osteoblast-specific factor 2 (OSF-2) from an MC3T3-E1 cDNA library using subtraction hybridization and differential screening techniques. Here we describe the localization, regulation, and potential function of this protein. Immunohistochemistry using specific antiserum revealed that in adult mice, the protein is preferentially expressed in periosteum and periodontal ligament, indicating its tissue specificity and a potential role in bone and tooth formation and maintenance of structure. Based on this observation and the fact that other proteins have been called OSF-2, the protein was renamed 'periostin.' Western blot analysis showed that periostin is a disulfide linked 90 kDa protein secreted by osteoblasts and osteoblast-like cell lines. Nucleotide sequence revealed four periostin transcripts that differ in the length of the C-terminal domain, possibly caused by alternative splicing events. Reverse transcription- polymerase chain reaction analysis revealed that these isoforms are not expressed uniformly but are differentially expressed in various cell lines. Both purified periostin protein and the periostin-Fc recombinant protein supported attachment and spreading of MC3T3- E1 cells, and this effect was impaired by antiperiostin antiserum, suggesting that periostin is involved in cell adhesion. The protein is highly homologous to βig-h3, a molecule induced by transforming growth factor β (TGF-β) that promotes the adhesion and spreading of fibroblasts. Because TGF-β has dramatic effects on periosteal expansion and the recruitment of osteoblast precursors, this factor was tested for its effects on periostin expression. By Western blot analysis, TGF-β increased periostin expression in primary osteoblast cells. Together, these data suggest that periostin may play a role in the recruitment and attachment of osteoblast precursors in the periosteum.

Original languageEnglish (US)
Pages (from-to)1239-1249
Number of pages11
JournalJournal of Bone and Mineral Research
Volume14
Issue number7
DOIs
StatePublished - 1999
Externally publishedYes

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Periosteum
Periodontal Ligament
Transforming Growth Factors
Osteoblasts
Proteins
Immune Sera
Western Blotting
Cell Line
Organ Specificity
Alternative Splicing
Gene Library
Recombinant Proteins
Osteogenesis
Cell Adhesion
Disulfides
Reverse Transcription
Tooth
Protein Isoforms
Complementary DNA
Fibroblasts

ASJC Scopus subject areas

  • Surgery

Cite this

Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor β. / Horiuchi, Keisuke; Amizuka, Norio; Takeshita, Sunao; Takamatsu, Hiroyuki; Katsuura, Mieko; Ozawa, Hidehiro; Toyama, Yoshiaki; Bonewald, Lynda; Kudo, Akira.

In: Journal of Bone and Mineral Research, Vol. 14, No. 7, 1999, p. 1239-1249.

Research output: Contribution to journalArticle

Horiuchi, Keisuke ; Amizuka, Norio ; Takeshita, Sunao ; Takamatsu, Hiroyuki ; Katsuura, Mieko ; Ozawa, Hidehiro ; Toyama, Yoshiaki ; Bonewald, Lynda ; Kudo, Akira. / Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor β. In: Journal of Bone and Mineral Research. 1999 ; Vol. 14, No. 7. pp. 1239-1249.
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AU - Takamatsu, Hiroyuki

AU - Katsuura, Mieko

AU - Ozawa, Hidehiro

AU - Toyama, Yoshiaki

AU - Bonewald, Lynda

AU - Kudo, Akira

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