Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer

Andrew Lindsley, Paige Snider, Hongming Zhou, Rhonda Rogers, Jian Wang, Michael Olaopa, Agnieszka Kruzynska-Frejtag, Shrinagesh V. Koushik, Brenda Lilly, John B E Burch, Anthony Firulli, Simon Conway

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Periostin is a fasciclin-containing adhesive glycoprotein that facilitates the migration and differentiation of cells that have undergone epithelial-mesenchymal transformation during embryogenesis and in pathological conditions. Despite the importance of post-transformational differentiation as a general developmental mechanism, little is known how periostin's embryonic expression is regulated. To help resolve this deficiency, a 3.9-kb periostin proximal promoter was isolated and shown to drive tissue-specific expression in the neural crest-derived Schwann cell lineage and in a subpopulation of periostin-expressing cells in the cardiac outflow tract endocardial cushions. In order to identify the enhancer and associated DNA binding factor(s) responsible, in vitro promoter dissection was undertaken in a Schwannoma line. Ultimately a 304-bpperi enhancer was identified and shown to be capable of recapitulating 3.9 kbperi-lacZ in vivo spatiotemporal patterns. Further mutational and EMSA analysis helped identify a minimal 37-bp region that is bound by the YY1 transcription factor. The 37-bp enhancer was subsequently shown to be essential for in vivo 3.9 kbperi-lacZ promoter activity. Taken together, these studies identify an evolutionary-conserved YY1-binding 37-bp region within a 304-bp periostin core enhancer that is capable of regulating simultaneous novel tissue-specific periostin expression in the cardiac outflow-tract cushion mesenchyme and Schwann cell lineages.

Original languageEnglish
Pages (from-to)340-355
Number of pages16
JournalDevelopmental Biology
Volume307
Issue number2
DOIs
StatePublished - Jul 15 2007

Fingerprint

Endocardial Cushions
Schwann Cells
Cell Lineage
YY1 Transcription Factor
Epithelial-Mesenchymal Transition
Neural Crest
Neurilemmoma
Mesoderm
Adhesives
Embryonic Development
Cell Movement
Dissection
Cell Differentiation
Glycoproteins
DNA

Keywords

  • Endocardial cushions
  • Heart development
  • lacZ reporter mice
  • Lineage restricted promoter
  • Mouse embryo
  • Periostin
  • Peripheral nervous system
  • Schwann cells

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer. / Lindsley, Andrew; Snider, Paige; Zhou, Hongming; Rogers, Rhonda; Wang, Jian; Olaopa, Michael; Kruzynska-Frejtag, Agnieszka; Koushik, Shrinagesh V.; Lilly, Brenda; Burch, John B E; Firulli, Anthony; Conway, Simon.

In: Developmental Biology, Vol. 307, No. 2, 15.07.2007, p. 340-355.

Research output: Contribution to journalArticle

Lindsley, A, Snider, P, Zhou, H, Rogers, R, Wang, J, Olaopa, M, Kruzynska-Frejtag, A, Koushik, SV, Lilly, B, Burch, JBE, Firulli, A & Conway, S 2007, 'Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer', Developmental Biology, vol. 307, no. 2, pp. 340-355. https://doi.org/10.1016/j.ydbio.2007.04.041
Lindsley, Andrew ; Snider, Paige ; Zhou, Hongming ; Rogers, Rhonda ; Wang, Jian ; Olaopa, Michael ; Kruzynska-Frejtag, Agnieszka ; Koushik, Shrinagesh V. ; Lilly, Brenda ; Burch, John B E ; Firulli, Anthony ; Conway, Simon. / Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer. In: Developmental Biology. 2007 ; Vol. 307, No. 2. pp. 340-355.
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