Identification and characterization of hemoangiogenic progenitors during cynomolgus monkey embryonic stem cell differentiation

Katsutsugu Umeda, Toshio Heike, Momoko Yoshimoto, Gen Shinoda, Mitsutaka Shiota, Hirofumi Suemori, Yuan Luo Hong, David H K Chui, Ryuzo Torii, Masabumi Shibuya, Norio Nakatsuji, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We identified intermediate-stage progenitor cells that have the potential to differentiate into hematopoietic and endothelial lineages from nonhuman primate embryonic stem (ES) cells. Sequential fluorescence-activated cell sorting and immunostaining analyses showed that when ES cells were cultured in an OP9 coculture system, both lineages developed after the emergence of two hemoangiogenic progenitor-bearing cell fractions, namely, vascular endothelial growth factor receptor (VEGFR)-2high CD34- and VEGFR-2high CD34+ cells. Exogenous vascular endothelial growth factor increased the proportion of VEGFR-2high cells, particularly that of VEGFR-2high CD34+ cells, in a dose-dependent manner. Although either population of VEGFR-2high cells could differentiate into primitive and definitive hematopoietic cells (HCs), as well as endothelial cells (ECs), the VEGFR-2high CD34 + cells had greater hemoangiogenic potential. Both lineages developed from VEGFR-2high CD34- or VEGFR-2high CD34+ precursor at the single-cell level, which strongly supports the existence of hemangioblasts in these cell fractions. Thus, this culture system allows differentiation into the HC and EC lineages to be defined by surface markers. These observations should facilitate further studies both on early developmental processes and on regeneration therapies in human.

Original languageEnglish (US)
Pages (from-to)1348-1358
Number of pages11
JournalStem Cells
Volume24
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

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Vascular Endothelial Growth Factor Receptor
Macaca fascicularis
Embryonic Stem Cells
Cell Differentiation
Hemangioblasts
Stem Cells
Endothelial Cells
Cell Lineage
Coculture Techniques
Primates
Vascular Endothelial Growth Factor A
Regeneration
Flow Cytometry

Keywords

  • Embryonic stem cell
  • Hemangioblast
  • Primate
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cell Biology

Cite this

Umeda, K., Heike, T., Yoshimoto, M., Shinoda, G., Shiota, M., Suemori, H., ... Nakahata, T. (2006). Identification and characterization of hemoangiogenic progenitors during cynomolgus monkey embryonic stem cell differentiation. Stem Cells, 24(5), 1348-1358. https://doi.org/10.1634/stemcells.2005-0165

Identification and characterization of hemoangiogenic progenitors during cynomolgus monkey embryonic stem cell differentiation. / Umeda, Katsutsugu; Heike, Toshio; Yoshimoto, Momoko; Shinoda, Gen; Shiota, Mitsutaka; Suemori, Hirofumi; Hong, Yuan Luo; Chui, David H K; Torii, Ryuzo; Shibuya, Masabumi; Nakatsuji, Norio; Nakahata, Tatsutoshi.

In: Stem Cells, Vol. 24, No. 5, 05.2006, p. 1348-1358.

Research output: Contribution to journalArticle

Umeda, K, Heike, T, Yoshimoto, M, Shinoda, G, Shiota, M, Suemori, H, Hong, YL, Chui, DHK, Torii, R, Shibuya, M, Nakatsuji, N & Nakahata, T 2006, 'Identification and characterization of hemoangiogenic progenitors during cynomolgus monkey embryonic stem cell differentiation', Stem Cells, vol. 24, no. 5, pp. 1348-1358. https://doi.org/10.1634/stemcells.2005-0165
Umeda, Katsutsugu ; Heike, Toshio ; Yoshimoto, Momoko ; Shinoda, Gen ; Shiota, Mitsutaka ; Suemori, Hirofumi ; Hong, Yuan Luo ; Chui, David H K ; Torii, Ryuzo ; Shibuya, Masabumi ; Nakatsuji, Norio ; Nakahata, Tatsutoshi. / Identification and characterization of hemoangiogenic progenitors during cynomolgus monkey embryonic stem cell differentiation. In: Stem Cells. 2006 ; Vol. 24, No. 5. pp. 1348-1358.
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