Identification and characterization of muscarinic receptors in cultured human pancreatic carcinoma cells

Martha S. Ackerman, William R. Roeske, Richard J. Heck, Murray Korc

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Five human pancreatic carcinoma cell lines were screened for the presence of muscarinic cholinergic receptors (mAChRs), using [3H]7V-meth-ylscopolamine ([3H]NMS). T3M4 and COLO-357 cells exhibited specific, high-affinity binding to mAChRs. A small amount of [3H]NMS also bound in PANC-I and ASPC-I cells, but not in MIA PaCa-2 cells. Atropine, pirenzepine (PZ), and ll-[[2-[(diethylamino) methyl]-1-piperidinyl] acetyl]-5, ll-dihydro-6H-pyrido-[2, 3-b][l, 4] benzodiazepine-6-one (AF-DX 116) inhibited [3H]NMS binding and carbachol-mediated [3H]inositol monophosphate formation in both T3M4 and COLO-357 cells. The order of inhibition was: Atropine > PZ > AF-DX 116. Carbachol did not alter [3H]inositol monophosphate formation in the other cell lines. These findings suggest that the mAChRs expressed in some human pancreatic cancer cells exhibit the pharmacologic characteristics of a muscarinic receptor subtype with an intermediate affinity for PZ and a lower affinity for AF-DX 116 and are functionally coupled to activation of phospholipid hydrolysis.

Original languageEnglish (US)
Pages (from-to)1-2
Number of pages2
JournalPancreas
Volume4
Issue number3
StatePublished - Jun 1989

Keywords

  • Muscarinic receptors
  • Pancreatic carcinomas
  • Phospholipid hydrolysis

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Fingerprint Dive into the research topics of 'Identification and characterization of muscarinic receptors in cultured human pancreatic carcinoma cells'. Together they form a unique fingerprint.

  • Cite this

    Ackerman, M. S., Roeske, W. R., Heck, R. J., & Korc, M. (1989). Identification and characterization of muscarinic receptors in cultured human pancreatic carcinoma cells. Pancreas, 4(3), 1-2.