Identification and disruption of a rhoptry-localized homologue of sodium hydrogen exchangers in Toxoplasma gondii

Ariela O. Karasov, John C. Boothroyd, Gustavo Arrizabalaga

Research output: Contribution to journalArticle

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Abstract

Na+/H+ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na+ for H+ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules.

Original languageEnglish (US)
Pages (from-to)285-291
Number of pages7
JournalInternational Journal for Parasitology
Volume35
Issue number3
DOIs
StatePublished - Mar 2005

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Keywords

  • Osmoregulation
  • Rhoptry
  • Sodium hydrogen exchanger
  • Toxoplasma

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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