Identification of 6H1 as a P2Y purinoceptor: P2Y5

Tania E. Webb, Mark G. Kaplan, Eric A. Barnard

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

We have determined the identity of the orphan G-protein coupled receptor cDNA, 6H1, present in activated chicken T cells, as a subtype of P2Y purinoceptor. This identification is based first on the degree of sequence identity shared with recently cloned members of the P2Y receptor family and second on the pharmacological profile. Upon transient expression in COS-7 cells the 6H1 receptor bound the radiolabel [35S]dATPαS specifically and with high affinity (Kd, 10 nM). This specific binding could be competitively displaced by a range of ligands active at P2 purinoceptors, with ATP being the most active (Ki, 116 nM). Such competition studies have established the following rank order of activity: ATP ADP 2-methylthioATP α,β-methyleneATP, UTP, thus confirming 6H1 as a member of the growing family of P2Y purinoceptors. As the fifth receptor of this type to be identified we suggest that it be named P2Y5.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume219
Issue number1
DOIs
StatePublished - Feb 6 1996

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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