Identification of a G protein coupled receptor induced in activated T cells

M. H. Kaplan, D. I. Smith, R. S. Sundick

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37 Scopus citations

Abstract

Many genes are induced after T cell activation to make a cell competent for proliferation and ultimately, function. Many of these genes encode surface receptors for growth factors that signal a cell to proliferate. We have cloned a novel gene (clone 6H1) that codes for a member of the G protein-coupled receptor superfamily. This gene was isolated from a chicken activated T cell cDNA library by low level hybridization to mammalian IL-2 cDNA probes. The 308 amino acid open reading frame has seven hydrophobic, presumably transmembrane domains and a consensus site for interaction with G proteins. Tissue distribution studies suggest that gene expression is restricted to activated T cells. The message appears by 1 h after activation and is maintained for at least 45 h. Transcription of 6H1 is induced by a number of T cell stimuli and is inhibited by cyclosporin A, but not by cycloheximide. This is the first description of a member of this superfamily expressed specifically in activated T cells. The gene product may provide a link between T cell growth factors and G protein activation.

Original languageEnglish (US)
Pages (from-to)628-636
Number of pages9
JournalJournal of Immunology
Volume151
Issue number2
StatePublished - Jan 1 1993

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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