Identification of a G Protein Coupled Receptor Induced in Activated T Cells

Mark Kaplan, David I. Smith, Roy S. Sundick

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Many genes are induced after T cell activation to make a cell competent for proliferation and ultimately, function. Many of these genes encode surface receptors for growth factors that signal a cell to proliferate. We have cloned a novel gene (clone 6H1) that codes for a member of the G protein-coupled receptor superfamily. This gene was isolated from a chicken activated T cell cDNA library by low level hybridization to mammalian IL-2 cDNA probes. The 308 amino acid open reading frame has seven hydrophobic, presumably transmembrane domains and a consensus site for interaction with G proteins. Tissue distribution studies suggest that gene expression is restricted to activated T cells. The message appears by 1 h after activation and is maintained for at least 45 h. Transcription of 6H1 is induced by a number of T cell stimuli and is inhibited by cyclosporin A, but not by cycloheximide. This is the first description of a member of this superfamily expressed specifically in activated T cells. The gene product may provide a link between T cell growth factors and G protein activation.

Original languageEnglish (US)
Pages (from-to)628-636
Number of pages9
JournalJournal of Immunology
Volume151
Issue number2
StatePublished - Jul 15 1993
Externally publishedYes

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G-Protein-Coupled Receptors
T-Lymphocytes
Genes
GTP-Binding Proteins
Interleukin-2
Growth Factor Receptors
Tissue Distribution
Cycloheximide
Gene Library
Cyclosporine
Open Reading Frames
Chickens
Complementary DNA
Clone Cells
Cell Proliferation
Gene Expression
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of a G Protein Coupled Receptor Induced in Activated T Cells. / Kaplan, Mark; Smith, David I.; Sundick, Roy S.

In: Journal of Immunology, Vol. 151, No. 2, 15.07.1993, p. 628-636.

Research output: Contribution to journalArticle

Kaplan, Mark ; Smith, David I. ; Sundick, Roy S. / Identification of a G Protein Coupled Receptor Induced in Activated T Cells. In: Journal of Immunology. 1993 ; Vol. 151, No. 2. pp. 628-636.
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