Identification of a high-molecular-weight (> 400 000) protein in hyaline cartilage

Rose Spitz Fife, Kenneth D. Brandt

Research output: Contribution to journalArticle

41 Scopus citations


A high-molecular-weight (> 400 000) non-collagenous protein has been identified in normal articular cartilage from several mammalian species and in bovine tracheal cartilage. This protein is reduced by 2-mercaptoethanol to subunits with a molecular weight of 116 000, which appear to constitute approx. 2-4% of the total protein detectable by the Lowry assay in 4 M guanidinium chloride extracts of normal bovine and canine articular cartilage. Antiserum to the 116 kDa subunit protein from bovine articular cartilage cross-reacts with the intact and subunit proteins from bovine trachea and from normal canine, porcine and human articular cartilage. This protein is not found in non-cartilagenous tissues, suggesting that it is a cartilage-specific protein. We conclude that the > 400 kDa protein and its subunit are ubiquitous and quantitatively significant proteins in hyaline cartilage.

Original languageEnglish (US)
Pages (from-to)506-514
Number of pages9
JournalBBA - General Subjects
Issue number3
StatePublished - Dec 20 1984


  • Cartilage
  • Non-collagen protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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