Identification of a high-molecular-weight (> 400 000) protein in hyaline cartilage

Rose Fife, Kenneth D. Brandt

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A high-molecular-weight (> 400 000) non-collagenous protein has been identified in normal articular cartilage from several mammalian species and in bovine tracheal cartilage. This protein is reduced by 2-mercaptoethanol to subunits with a molecular weight of 116 000, which appear to constitute approx. 2-4% of the total protein detectable by the Lowry assay in 4 M guanidinium chloride extracts of normal bovine and canine articular cartilage. Antiserum to the 116 kDa subunit protein from bovine articular cartilage cross-reacts with the intact and subunit proteins from bovine trachea and from normal canine, porcine and human articular cartilage. This protein is not found in non-cartilagenous tissues, suggesting that it is a cartilage-specific protein. We conclude that the > 400 kDa protein and its subunit are ubiquitous and quantitatively significant proteins in hyaline cartilage.

Original languageEnglish
Pages (from-to)506-514
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume802
Issue number3
DOIs
StatePublished - Dec 20 1984

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Hyaline Cartilage
Cartilage
Molecular Weight
Molecular weight
Articular Cartilage
Protein Subunits
Proteins
Canidae
Mercaptoethanol
Guanidine
Trachea
Immune Sera
Swine
Assays
Tissue

Keywords

  • Cartilage
  • Non-collagen protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a high-molecular-weight (> 400 000) protein in hyaline cartilage. / Fife, Rose; Brandt, Kenneth D.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 802, No. 3, 20.12.1984, p. 506-514.

Research output: Contribution to journalArticle

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