Identification of a human CDNA encoding a novel transcription factor

Lu Zhang, Jinghua Zhao, Howard Edenberg

Research output: Contribution to journalArticle

Abstract

In an effort to search for transcription factors involved in ADH gene expression, we have examined the effects of multimers of individual cis-arting elements inserted in front of an SV40 early promoter in the iuciferase expression vector pGL2-Promoter (Promega). A trimer of a ubiquitous site in ADH3 (for which we had not identified a known transcription factor) showed weak enhancer-like activity, increasing transcription 1.5- to 2-fold regardless of tho orientation in which it was inserted. Using this trimer as ligand to screen a human liver cDNA expression library, we identified a positive clone which appears to be a novel transcription factor. Competition studies suggests that the binding to the ligand is sequence specific. We rescreened the human liver cDNA library and identified nine overlapping clones representing 3.-4 kb of cDNA. The nucleotide sequence of the coding region has extremely high G-I-C content (75.5%). This cDNA is not closely related to any sequence in the GenBank database, except for a region that contains Iwo tandem C2 H2 zinc fingers that match the consensus well. The conserved zinc finger motif probably serves as a DNA binding domain. The deduced amino acid sequence of this putative transcription factor contains a very hydrophilic region, and ends with a cluster of stop signals. [Supported by N.I.A.A.A. R01-AA06460.].

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

Forensic Anthropology
Transcription Factors
transcription factors
Complementary DNA
zinc finger motif
Zinc Fingers
Gene Library
Liver
Zinc
National Institute on Alcohol Abuse and Alcoholism (U.S.)
Clone Cells
promoter regions
clones
Ligands
liver
DNA-binding domains
Nucleic Acid Databases
Base Composition
Transcription
Gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Identification of a human CDNA encoding a novel transcription factor. / Zhang, Lu; Zhao, Jinghua; Edenberg, Howard.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

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