Identification of a ligand for the c-kit proto-oncogene

Douglas E. Williams, June Eisenman, Allison Baird, Charles Rauch, Kirk Van Ness, Carl J. March, Linda S. Park, Unja Martin, Diane Y. Mochizukl, H. Scott Boswell, Gem S. Burgess, David Cosman, Stewart D. Lyman

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Abstract

We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalCell
Volume63
Issue number1
DOIs
StatePublished - Oct 5 1990

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Williams, D. E., Eisenman, J., Baird, A., Rauch, C., Van Ness, K., March, C. J., Park, L. S., Martin, U., Mochizukl, D. Y., Boswell, H. S., Burgess, G. S., Cosman, D., & Lyman, S. D. (1990). Identification of a ligand for the c-kit proto-oncogene. Cell, 63(1), 167-174. https://doi.org/10.1016/0092-8674(90)90297-R