Identification of a ligand for the c-kit proto-oncogene

Douglas E. Williams; June Eisenman; Allison Baird; Charles Rauch; Kirk Van Ness; Carl J. March; Linda S. Park; Unja Martin; Diane Y. Mochizukl; H. Scott Boswell; Gem S. Burgess; David Cosman; Stewart D. Lyman

doi:10.1016/0092-8674(90)90297-R

Identification of a ligand for the c-kit proto-oncogene

Douglas E. Williams, June Eisenman, Allison Baird, Charles Rauch, Kirk Van Ness, Carl J. March, Linda S. Park, Unja Martin, Diane Y. Mochizukl, H. Scott Boswell, Gem S. Burgess, David Cosman, Stewart D. Lyman

Hematology/Oncology

Research output: Contribution to journal › Article

718 Scopus citations

Abstract

We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [³H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking ¹²⁵I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.

Original language	English (US)
Pages (from-to)	167-174
Number of pages	8
Journal	Cell
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(90)90297-R
State	Published - Oct 5 1990

Fingerprint

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Cite this

Williams, D. E., Eisenman, J., Baird, A., Rauch, C., Van Ness, K., March, C. J., Park, L. S., Martin, U., Mochizukl, D. Y., Boswell, H. S., Burgess, G. S., Cosman, D., & Lyman, S. D. (1990). Identification of a ligand for the c-kit proto-oncogene. Cell, 63(1), 167-174. https://doi.org/10.1016/0092-8674(90)90297-R

Identification of a ligand for the c-kit proto-oncogene. / Williams, Douglas E.; Eisenman, June; Baird, Allison; Rauch, Charles; Van Ness, Kirk; March, Carl J.; Park, Linda S.; Martin, Unja; Mochizukl, Diane Y.; Boswell, H. Scott; Burgess, Gem S.; Cosman, David; Lyman, Stewart D.

In: Cell, Vol. 63, No. 1, 05.10.1990, p. 167-174.

Research output: Contribution to journal › Article

@article{f622934a79294f7b8887a8917a9d8d84,

title = "Identification of a ligand for the c-kit proto-oncogene",

abstract = "We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.",

author = "Williams, {Douglas E.} and June Eisenman and Allison Baird and Charles Rauch and {Van Ness}, Kirk and March, {Carl J.} and Park, {Linda S.} and Unja Martin and Mochizukl, {Diane Y.} and Boswell, {H. Scott} and Burgess, {Gem S.} and David Cosman and Lyman, {Stewart D.}",

year = "1990",

month = oct,

day = "5",

doi = "10.1016/0092-8674(90)90297-R",

language = "English (US)",

volume = "63",

pages = "167--174",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Identification of a ligand for the c-kit proto-oncogene

AU - Williams, Douglas E.

AU - Eisenman, June

AU - Baird, Allison

AU - Rauch, Charles

AU - Van Ness, Kirk

AU - March, Carl J.

AU - Park, Linda S.

AU - Martin, Unja

AU - Mochizukl, Diane Y.

AU - Boswell, H. Scott

AU - Burgess, Gem S.

AU - Cosman, David

AU - Lyman, Stewart D.

PY - 1990/10/5

Y1 - 1990/10/5

N2 - We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.

AB - We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.

UR - http://www.scopus.com/inward/record.url?scp=0025013548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025013548&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(90)90297-R

DO - 10.1016/0092-8674(90)90297-R

M3 - Article

C2 - 1698553

AN - SCOPUS:0025013548

VL - 63

SP - 167

EP - 174

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -

Access to Document

10.1016/0092-8674(90)90297-R