Identification of a ligand for the c-kit proto-oncogene

Douglas E. Williams, June Eisenman, Allison Baird, Charles Rauch, Kirk Van Ness, Carl J. March, Linda S. Park, Unja Martin, Diane Y. Mochizukl, H. Boswell, Gem S. Burgess, David Cosman, Stewart D. Lyman

Research output: Contribution to journalArticle

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Abstract

We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalCell
Volume63
Issue number1
DOIs
StatePublished - Oct 5 1990

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Stem Cell Factor
Proto-Oncogenes
Cells
Proto-Oncogene Proteins c-kit
Cell Line
Interleukin-3
Stromal Cells
Immunoprecipitation
Thymidine
Purification
Immune Sera
Amino Acid Sequence
Assays
Ligands
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Williams, D. E., Eisenman, J., Baird, A., Rauch, C., Van Ness, K., March, C. J., ... Lyman, S. D. (1990). Identification of a ligand for the c-kit proto-oncogene. Cell, 63(1), 167-174. https://doi.org/10.1016/0092-8674(90)90297-R

Identification of a ligand for the c-kit proto-oncogene. / Williams, Douglas E.; Eisenman, June; Baird, Allison; Rauch, Charles; Van Ness, Kirk; March, Carl J.; Park, Linda S.; Martin, Unja; Mochizukl, Diane Y.; Boswell, H.; Burgess, Gem S.; Cosman, David; Lyman, Stewart D.

In: Cell, Vol. 63, No. 1, 05.10.1990, p. 167-174.

Research output: Contribution to journalArticle

Williams, DE, Eisenman, J, Baird, A, Rauch, C, Van Ness, K, March, CJ, Park, LS, Martin, U, Mochizukl, DY, Boswell, H, Burgess, GS, Cosman, D & Lyman, SD 1990, 'Identification of a ligand for the c-kit proto-oncogene', Cell, vol. 63, no. 1, pp. 167-174. https://doi.org/10.1016/0092-8674(90)90297-R
Williams DE, Eisenman J, Baird A, Rauch C, Van Ness K, March CJ et al. Identification of a ligand for the c-kit proto-oncogene. Cell. 1990 Oct 5;63(1):167-174. https://doi.org/10.1016/0092-8674(90)90297-R
Williams, Douglas E. ; Eisenman, June ; Baird, Allison ; Rauch, Charles ; Van Ness, Kirk ; March, Carl J. ; Park, Linda S. ; Martin, Unja ; Mochizukl, Diane Y. ; Boswell, H. ; Burgess, Gem S. ; Cosman, David ; Lyman, Stewart D. / Identification of a ligand for the c-kit proto-oncogene. In: Cell. 1990 ; Vol. 63, No. 1. pp. 167-174.
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