Abstract
We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.
| Original language | English |
|---|---|
| Pages (from-to) | 167-174 |
| Number of pages | 8 |
| Journal | Cell |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 5 1990 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Identification of a ligand for the c-kit proto-oncogene. / Williams, Douglas E.; Eisenman, June; Baird, Allison; Rauch, Charles; Van Ness, Kirk; March, Carl J.; Park, Linda S.; Martin, Unja; Mochizukl, Diane Y.; Boswell, H.; Burgess, Gem S.; Cosman, David; Lyman, Stewart D.
In: Cell, Vol. 63, No. 1, 05.10.1990, p. 167-174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a ligand for the c-kit proto-oncogene
AU - Williams, Douglas E.
AU - Eisenman, June
AU - Baird, Allison
AU - Rauch, Charles
AU - Van Ness, Kirk
AU - March, Carl J.
AU - Park, Linda S.
AU - Martin, Unja
AU - Mochizukl, Diane Y.
AU - Boswell, H.
AU - Burgess, Gem S.
AU - Cosman, David
AU - Lyman, Stewart D.
PY - 1990/10/5
Y1 - 1990/10/5
N2 - We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.
AB - We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.
UR - http://www.scopus.com/inward/record.url?scp=0025013548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025013548&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(90)90297-R
DO - 10.1016/0092-8674(90)90297-R
M3 - Article
VL - 63
SP - 167
EP - 174
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -