Identification of a Locus on Mouse Chromosome 17 Associated with High-Affinity Choline Uptake Using BXD Recombinant Inbred Mice and Quantitative Trait Loci Analysis

Bonnie J. Tarricone, Wayne G. Hwang, Joseph N. Hingtgen, Steve R. Mitchell, John K. Belknap, John Nurnberger

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Abstract

Using the quantitative trait loci (QTL) approach, preliminary identification has been made of a region on mouse chromosome 17 that influences high-affinity choline uptake (HACU) in the mouse brain. The rate of HACU was measured in synaptosomes prepared from the frontal cortex, hippocampus, and striatum of C57BL/6J (B6), DBA/2J (D2), and 25 BXD recombinant inbred (RI) strains of mice, using a final concentration of 0.5 μM [3H]choline. The strain means of HACU in each area were then correlated with the strain distribution pattern of each of 1300 known genetic markers using a point biserial correlation and 0 (B6 allele) and 1 (D2 allele). Correlations of P < 0.00001 were found between striatal HACU and chromosome 17 markers D17Tu50 and Tcp1. Correlations of P < 0.0001 were found between striatal HACU and chromosome 17 markers D17Leh66e, D17Leh119, D17Rp17e, Plg, D17Leh66d, Ckb-rs2, and Trp53-ps. QTL analyses of HACU in the frontal cortex and hippocampus also revealed correlations with these markers at the level of P < 0.05 and P < 0.01. These data suggest that at least one locus located on mouse chromosome 17 near or between 6 and 13 cM from the centromere influences HACU in the striatum and possibly the frontal cortex and hippocampus of the mouse.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalGenomics
Volume27
Issue number1
DOIs
StatePublished - May 1 1995

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Chromosomes, Human, Pair 17
Quantitative Trait Loci
Choline
Frontal Lobe
Genetic Markers
Corpus Striatum
Hippocampus
Alleles
Inbred Strains Mice
Synaptosomes
Centromere
Brain

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of a Locus on Mouse Chromosome 17 Associated with High-Affinity Choline Uptake Using BXD Recombinant Inbred Mice and Quantitative Trait Loci Analysis. / Tarricone, Bonnie J.; Hwang, Wayne G.; Hingtgen, Joseph N.; Mitchell, Steve R.; Belknap, John K.; Nurnberger, John.

In: Genomics, Vol. 27, No. 1, 01.05.1995, p. 161-164.

Research output: Contribution to journalArticle

Tarricone, Bonnie J. ; Hwang, Wayne G. ; Hingtgen, Joseph N. ; Mitchell, Steve R. ; Belknap, John K. ; Nurnberger, John. / Identification of a Locus on Mouse Chromosome 17 Associated with High-Affinity Choline Uptake Using BXD Recombinant Inbred Mice and Quantitative Trait Loci Analysis. In: Genomics. 1995 ; Vol. 27, No. 1. pp. 161-164.
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abstract = "Using the quantitative trait loci (QTL) approach, preliminary identification has been made of a region on mouse chromosome 17 that influences high-affinity choline uptake (HACU) in the mouse brain. The rate of HACU was measured in synaptosomes prepared from the frontal cortex, hippocampus, and striatum of C57BL/6J (B6), DBA/2J (D2), and 25 BXD recombinant inbred (RI) strains of mice, using a final concentration of 0.5 μM [3H]choline. The strain means of HACU in each area were then correlated with the strain distribution pattern of each of 1300 known genetic markers using a point biserial correlation and 0 (B6 allele) and 1 (D2 allele). Correlations of P < 0.00001 were found between striatal HACU and chromosome 17 markers D17Tu50 and Tcp1. Correlations of P < 0.0001 were found between striatal HACU and chromosome 17 markers D17Leh66e, D17Leh119, D17Rp17e, Plg, D17Leh66d, Ckb-rs2, and Trp53-ps. QTL analyses of HACU in the frontal cortex and hippocampus also revealed correlations with these markers at the level of P < 0.05 and P < 0.01. These data suggest that at least one locus located on mouse chromosome 17 near or between 6 and 13 cM from the centromere influences HACU in the striatum and possibly the frontal cortex and hippocampus of the mouse.",
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