Identification of a new interferon-α/β-inducible DNA-binding protein that interacts with the regulatory element A of 2',5'-oligoadenylate synthetase ME-12 gene

Cong Yan, I. Tamm

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new interferon (IFN)-stimulated response factor (ISRF) has been identified in nuclear extracts of IFN-α/β-treated murine BALB/c-3T3 fibroblasts by the mobility-shift electrophoresis assay. The factor, ISRF-2, displays murine 2',5'-oligoadenylate (2-5A) synthetase ME-12 gene 5' regulatory element A specificity and differs from the previously described IFN response element B-specific factor ISRF-1 in several aspects. ISRF-2 is restricted to the nucleus, whereas ISRF-1 exists in the cytoplasm and translocates into the nucleus upon treatment of cells with IFN-α/β. The ionic strength requirement of ISRF-2 for maximal DNA-binding activity is lower than that of ISRF-1. The DNA-binding activity of ISRF-2, but not that of ISRF-1, is markedly suppressed by Mg2+. In common with ISRF-1, the phosphorylated form of ISRF-2 appears to be required for DNA-binding activity. A model is proposed for the mechanism whereby murine IFN-α/β regulates 2-5A synthetase ME-12 gene expression.

Original languageEnglish (US)
Pages (from-to)425-430
Number of pages6
JournalJournal of Interferon Research
Volume12
Issue number6
StatePublished - 1992
Externally publishedYes

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2',5'-Oligoadenylate Synthetase
DNA-Binding Proteins
Interferons
Genes
DNA
Response Elements
Electrophoretic Mobility Shift Assay
Regulator Genes
Osmolar Concentration
Electrophoresis
Cytoplasm
Fibroblasts
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

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title = "Identification of a new interferon-α/β-inducible DNA-binding protein that interacts with the regulatory element A of 2',5'-oligoadenylate synthetase ME-12 gene",
abstract = "A new interferon (IFN)-stimulated response factor (ISRF) has been identified in nuclear extracts of IFN-α/β-treated murine BALB/c-3T3 fibroblasts by the mobility-shift electrophoresis assay. The factor, ISRF-2, displays murine 2',5'-oligoadenylate (2-5A) synthetase ME-12 gene 5' regulatory element A specificity and differs from the previously described IFN response element B-specific factor ISRF-1 in several aspects. ISRF-2 is restricted to the nucleus, whereas ISRF-1 exists in the cytoplasm and translocates into the nucleus upon treatment of cells with IFN-α/β. The ionic strength requirement of ISRF-2 for maximal DNA-binding activity is lower than that of ISRF-1. The DNA-binding activity of ISRF-2, but not that of ISRF-1, is markedly suppressed by Mg2+. In common with ISRF-1, the phosphorylated form of ISRF-2 appears to be required for DNA-binding activity. A model is proposed for the mechanism whereby murine IFN-α/β regulates 2-5A synthetase ME-12 gene expression.",
author = "Cong Yan and I. Tamm",
year = "1992",
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TY - JOUR

T1 - Identification of a new interferon-α/β-inducible DNA-binding protein that interacts with the regulatory element A of 2',5'-oligoadenylate synthetase ME-12 gene

AU - Yan, Cong

AU - Tamm, I.

PY - 1992

Y1 - 1992

N2 - A new interferon (IFN)-stimulated response factor (ISRF) has been identified in nuclear extracts of IFN-α/β-treated murine BALB/c-3T3 fibroblasts by the mobility-shift electrophoresis assay. The factor, ISRF-2, displays murine 2',5'-oligoadenylate (2-5A) synthetase ME-12 gene 5' regulatory element A specificity and differs from the previously described IFN response element B-specific factor ISRF-1 in several aspects. ISRF-2 is restricted to the nucleus, whereas ISRF-1 exists in the cytoplasm and translocates into the nucleus upon treatment of cells with IFN-α/β. The ionic strength requirement of ISRF-2 for maximal DNA-binding activity is lower than that of ISRF-1. The DNA-binding activity of ISRF-2, but not that of ISRF-1, is markedly suppressed by Mg2+. In common with ISRF-1, the phosphorylated form of ISRF-2 appears to be required for DNA-binding activity. A model is proposed for the mechanism whereby murine IFN-α/β regulates 2-5A synthetase ME-12 gene expression.

AB - A new interferon (IFN)-stimulated response factor (ISRF) has been identified in nuclear extracts of IFN-α/β-treated murine BALB/c-3T3 fibroblasts by the mobility-shift electrophoresis assay. The factor, ISRF-2, displays murine 2',5'-oligoadenylate (2-5A) synthetase ME-12 gene 5' regulatory element A specificity and differs from the previously described IFN response element B-specific factor ISRF-1 in several aspects. ISRF-2 is restricted to the nucleus, whereas ISRF-1 exists in the cytoplasm and translocates into the nucleus upon treatment of cells with IFN-α/β. The ionic strength requirement of ISRF-2 for maximal DNA-binding activity is lower than that of ISRF-1. The DNA-binding activity of ISRF-2, but not that of ISRF-1, is markedly suppressed by Mg2+. In common with ISRF-1, the phosphorylated form of ISRF-2 appears to be required for DNA-binding activity. A model is proposed for the mechanism whereby murine IFN-α/β regulates 2-5A synthetase ME-12 gene expression.

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