Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood

David Ingram, Laura E. Mead, Hiromi Tanaka, Virginia Meade, Amy Fenoglio, Kelly Mortell, Karen Pollok, Michael J. Ferkowicz, David Gilley, Mervin Yoder

Research output: Contribution to journalArticle

1088 Citations (Scopus)

Abstract

Emerging evidence to support the use of endothelial progenitor cells (EPCs) for angiogenic therapies or as biomarkers to assess cardiovascular disease risk and progression is compelling. However, there is no uniform definition of an EPC, which makes interpretation of these studies difficult. Although hallmarks of stem and progenitor cells are their ability to proliferate and to give rise to functional progeny, EPCs are primarily defined by the expression of cell-surface antigens. Here, using adult peripheral and umbilical cord blood, we describe an approach that identifies a novel hierarchy of EPCs based on their clonogenic and proliferates potential, analogous to the hematopoietic cell system. In fact, some EPCs form replatable colonies when deposited at the single-cell level. Using this approach, we also identify a previously unrecognised population of EPCs in cord blood that can achieve at least 100 population doublings, replate into at least secondary and tertiary colonies, and retain high levels of telomerase activity. Thus, these studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferative potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood.

Original languageEnglish
Pages (from-to)2752-2760
Number of pages9
JournalBlood
Volume104
Issue number9
DOIs
StatePublished - Nov 1 2004

Fingerprint

Endothelial cells
Fetal Blood
Blood
Stem Cells
Population
Hematopoietic System
Telomerase
Biomarkers
Surface Antigens
Cell- and Tissue-Based Therapy
Endothelial Progenitor Cells
Disease Progression
Cardiovascular Diseases
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. / Ingram, David; Mead, Laura E.; Tanaka, Hiromi; Meade, Virginia; Fenoglio, Amy; Mortell, Kelly; Pollok, Karen; Ferkowicz, Michael J.; Gilley, David; Yoder, Mervin.

In: Blood, Vol. 104, No. 9, 01.11.2004, p. 2752-2760.

Research output: Contribution to journalArticle

Ingram, David ; Mead, Laura E. ; Tanaka, Hiromi ; Meade, Virginia ; Fenoglio, Amy ; Mortell, Kelly ; Pollok, Karen ; Ferkowicz, Michael J. ; Gilley, David ; Yoder, Mervin. / Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. In: Blood. 2004 ; Vol. 104, No. 9. pp. 2752-2760.
@article{75482af05b7c4a2fbea080c19aa6a4c1,
title = "Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood",
abstract = "Emerging evidence to support the use of endothelial progenitor cells (EPCs) for angiogenic therapies or as biomarkers to assess cardiovascular disease risk and progression is compelling. However, there is no uniform definition of an EPC, which makes interpretation of these studies difficult. Although hallmarks of stem and progenitor cells are their ability to proliferate and to give rise to functional progeny, EPCs are primarily defined by the expression of cell-surface antigens. Here, using adult peripheral and umbilical cord blood, we describe an approach that identifies a novel hierarchy of EPCs based on their clonogenic and proliferates potential, analogous to the hematopoietic cell system. In fact, some EPCs form replatable colonies when deposited at the single-cell level. Using this approach, we also identify a previously unrecognised population of EPCs in cord blood that can achieve at least 100 population doublings, replate into at least secondary and tertiary colonies, and retain high levels of telomerase activity. Thus, these studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferative potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood.",
author = "David Ingram and Mead, {Laura E.} and Hiromi Tanaka and Virginia Meade and Amy Fenoglio and Kelly Mortell and Karen Pollok and Ferkowicz, {Michael J.} and David Gilley and Mervin Yoder",
year = "2004",
month = "11",
day = "1",
doi = "10.1182/blood-2004-04-1396",
language = "English",
volume = "104",
pages = "2752--2760",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "9",

}

TY - JOUR

T1 - Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood

AU - Ingram, David

AU - Mead, Laura E.

AU - Tanaka, Hiromi

AU - Meade, Virginia

AU - Fenoglio, Amy

AU - Mortell, Kelly

AU - Pollok, Karen

AU - Ferkowicz, Michael J.

AU - Gilley, David

AU - Yoder, Mervin

PY - 2004/11/1

Y1 - 2004/11/1

N2 - Emerging evidence to support the use of endothelial progenitor cells (EPCs) for angiogenic therapies or as biomarkers to assess cardiovascular disease risk and progression is compelling. However, there is no uniform definition of an EPC, which makes interpretation of these studies difficult. Although hallmarks of stem and progenitor cells are their ability to proliferate and to give rise to functional progeny, EPCs are primarily defined by the expression of cell-surface antigens. Here, using adult peripheral and umbilical cord blood, we describe an approach that identifies a novel hierarchy of EPCs based on their clonogenic and proliferates potential, analogous to the hematopoietic cell system. In fact, some EPCs form replatable colonies when deposited at the single-cell level. Using this approach, we also identify a previously unrecognised population of EPCs in cord blood that can achieve at least 100 population doublings, replate into at least secondary and tertiary colonies, and retain high levels of telomerase activity. Thus, these studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferative potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood.

AB - Emerging evidence to support the use of endothelial progenitor cells (EPCs) for angiogenic therapies or as biomarkers to assess cardiovascular disease risk and progression is compelling. However, there is no uniform definition of an EPC, which makes interpretation of these studies difficult. Although hallmarks of stem and progenitor cells are their ability to proliferate and to give rise to functional progeny, EPCs are primarily defined by the expression of cell-surface antigens. Here, using adult peripheral and umbilical cord blood, we describe an approach that identifies a novel hierarchy of EPCs based on their clonogenic and proliferates potential, analogous to the hematopoietic cell system. In fact, some EPCs form replatable colonies when deposited at the single-cell level. Using this approach, we also identify a previously unrecognised population of EPCs in cord blood that can achieve at least 100 population doublings, replate into at least secondary and tertiary colonies, and retain high levels of telomerase activity. Thus, these studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferative potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood.

UR - http://www.scopus.com/inward/record.url?scp=7244242362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7244242362&partnerID=8YFLogxK

U2 - 10.1182/blood-2004-04-1396

DO - 10.1182/blood-2004-04-1396

M3 - Article

VL - 104

SP - 2752

EP - 2760

JO - Blood

JF - Blood

SN - 0006-4971

IS - 9

ER -