Identification of a novel PP2C-type mitochondrial phosphatase

Mandar Joshi, Nam Ho Jeoung, Kirill M. Popov, Robert Harris

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A novel phosphatase has been cloned and partially characterized. It has a mitochondrial leader sequence and its amino acid sequence places it in the PP2C family like two known mitochondrial phosphatases. Western blot analysis of subcellular fractions and confocal microscopy of 3T3L1 preadipocytes expressing the GFP-tagged protein confirm its mitochondrial localization. Western blot analysis indicates that the protein is expressed in several mouse tissues, with highest expression in brain, heart, liver, and kidney. The recombinant protein exhibits Mn2+-dependent phosphoserine phosphatase activity against the branched-chain α-keto acid dehydrogenase complex, suggesting the enzyme may play a role in regulation of branched chain amino acid catabolism. Whether there are other mitochondrial substrates for the enzyme is not known.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number1
DOIs
StatePublished - Apr 27 2007

Fingerprint

Phosphoric Monoester Hydrolases
Western Blotting
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Branched Chain Amino Acids
Subcellular Fractions
Confocal microscopy
Enzymes
Recombinant Proteins
Confocal Microscopy
Liver
Amino Acid Sequence
Brain
Proteins
Tissue
Kidney
Amino Acids
Substrates
phosphoserine phosphatase

Keywords

  • Branched-chain α-keto acid dehydrogenase
  • Mitochondria
  • Protein phosphatase 2C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a novel PP2C-type mitochondrial phosphatase. / Joshi, Mandar; Jeoung, Nam Ho; Popov, Kirill M.; Harris, Robert.

In: Biochemical and Biophysical Research Communications, Vol. 356, No. 1, 27.04.2007, p. 38-44.

Research output: Contribution to journalArticle

Joshi, Mandar ; Jeoung, Nam Ho ; Popov, Kirill M. ; Harris, Robert. / Identification of a novel PP2C-type mitochondrial phosphatase. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 356, No. 1. pp. 38-44.
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