Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas

Jie Li, Chunyue Yin, Hiroaki Okamoto, Brian M. Balgley, Cheng S. Lee, Kristy Yuan, Barbara Ikejiri, Sven Glasker, Alexander Vortmeyer, Edward H. Oldfield, Robert J. Weil, Zhengping Zhuang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Dynamic changes in the expression of multiple genes appear to be common features that distinguish transformed cells from their normal counterparts. We compared the proteomic profiles of four glioblastoma multiforme (GBM) tissue samples and four normal brain cortex samples to examine the molecular basis of gliomagenesis. Trypsin-digested protein samples were separated by capillary isoelectric focusing with nano-reversed-phase liquid chromatography and were profiled by mass spectrometric sequencing. Wolf-Hirschhorn syndrome candidate 1 (WHSC1), along with 103 other proteins, was found only in the GBM proteomes. Western blot and immunohistochemistry verified our proteomic findings and demonstrated that 30-kDa WHSC1 expression increases with ascending tumor proliferation activity. RNA interference could suppress glioma cell growth by blocking WHSC1 expression. Our novel findings encourage the application of proteomic techniques in cancer research.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalNeuro-Oncology
Volume10
Issue number1
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Fingerprint

Wolf-Hirschhorn Syndrome
Glioma
Proteomics
Glioblastoma
Proteins
Isoelectric Focusing
Reverse-Phase Chromatography
Proteome
RNA Interference
Trypsin
Neoplasms
Western Blotting
Immunohistochemistry
Gene Expression
Brain
Growth
Research

Keywords

  • Expression
  • Glioma
  • Proliferation
  • Proteomic profiling
  • WHSC1

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Li, J., Yin, C., Okamoto, H., Balgley, B. M., Lee, C. S., Yuan, K., ... Zhuang, Z. (2008). Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. Neuro-Oncology, 10(1), 45-51. https://doi.org/10.1215/15228517-2007-036

Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. / Li, Jie; Yin, Chunyue; Okamoto, Hiroaki; Balgley, Brian M.; Lee, Cheng S.; Yuan, Kristy; Ikejiri, Barbara; Glasker, Sven; Vortmeyer, Alexander; Oldfield, Edward H.; Weil, Robert J.; Zhuang, Zhengping.

In: Neuro-Oncology, Vol. 10, No. 1, 01.02.2008, p. 45-51.

Research output: Contribution to journalArticle

Li, J, Yin, C, Okamoto, H, Balgley, BM, Lee, CS, Yuan, K, Ikejiri, B, Glasker, S, Vortmeyer, A, Oldfield, EH, Weil, RJ & Zhuang, Z 2008, 'Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas', Neuro-Oncology, vol. 10, no. 1, pp. 45-51. https://doi.org/10.1215/15228517-2007-036
Li, Jie ; Yin, Chunyue ; Okamoto, Hiroaki ; Balgley, Brian M. ; Lee, Cheng S. ; Yuan, Kristy ; Ikejiri, Barbara ; Glasker, Sven ; Vortmeyer, Alexander ; Oldfield, Edward H. ; Weil, Robert J. ; Zhuang, Zhengping. / Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. In: Neuro-Oncology. 2008 ; Vol. 10, No. 1. pp. 45-51.
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