Identification of a peptoid inhibitor of the proteasome 19S regulatory particle

Hyun-Suk Lim, Chase T. Archer, Thomas Kodadek

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The first chemical inhibitor of the 19S regulatory particle (RP) of the proteasome is described. The molecule was identified by screening a library of nucleoside-capped peptoids for binding to the yeast 26S proteasome in a crude extract. The hit was resynthesized and shown to block 19S RP-mediated protein unfolding in vitro and proteasome-mediated turnover of p27 in HeLa cells.

Original languageEnglish (US)
Pages (from-to)7750-7751
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number25
DOIs
StatePublished - Jun 27 2007
Externally publishedYes

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Peptoids
Proteasome Inhibitors
Proteasome Endopeptidase Complex
Protein Unfolding
Complex Mixtures
HeLa Cells
Nucleosides
Yeast
Screening
Yeasts
Molecules
Proteins

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Identification of a peptoid inhibitor of the proteasome 19S regulatory particle. / Lim, Hyun-Suk; Archer, Chase T.; Kodadek, Thomas.

In: Journal of the American Chemical Society, Vol. 129, No. 25, 27.06.2007, p. 7750-7751.

Research output: Contribution to journalArticle

Lim, Hyun-Suk ; Archer, Chase T. ; Kodadek, Thomas. / Identification of a peptoid inhibitor of the proteasome 19S regulatory particle. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 25. pp. 7750-7751.
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