Identification of a tap-dependent leader peptide recognized by alloreactive T cells specific for a class Ib antigen

Carla Aldrich, Amy DeCloux, Amina S. Woods, Robert J. Cotter, Mark J. Soloski, James Forman

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

Recognition of the class Ib antigen Qa-1 by a portion of alloreactive cytotoxic T lymphocyte (CTL) clones requires that the target cell express a second gene, termed Qa-1 determinant modifier (Qdm). We show that Qdm is identical to most D allele genes, excepting Dk, and that a nonamer peptide derived from D alloantigens restores CTL recognition on cells that lack the Qdm-encoded determinant. The equivalent Dk peptide has an Ala→Val interchange at P3 and requires ∼4 logs more peptide than the AlaP3 peptide for target cell lysis. Two of five CTL clones, not dependent on Qdm for target cell recognition, also recognize the Qdm peptide as well as the ValP3 variant. Although the Qdm peptide spans residues 3-11 from the leader, it requires the Tap transporters for its expression. Thus, the response against this class Ib molecule provides a tool for dissecting alloreactivity as well as pathways for antigen presentation.

Original languageEnglish (US)
Pages (from-to)649-658
Number of pages10
JournalCell
Volume79
Issue number4
DOIs
StatePublished - Nov 18 1994
Externally publishedYes

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T-cells
Protein Sorting Signals
T-Lymphocytes
Antigens
Peptides
Cytotoxic T-Lymphocytes
Clone Cells
Genes
Isoantigens
Antigen Presentation
Interchanges
Alleles
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Identification of a tap-dependent leader peptide recognized by alloreactive T cells specific for a class Ib antigen. / Aldrich, Carla; DeCloux, Amy; Woods, Amina S.; Cotter, Robert J.; Soloski, Mark J.; Forman, James.

In: Cell, Vol. 79, No. 4, 18.11.1994, p. 649-658.

Research output: Contribution to journalArticle

Aldrich, Carla ; DeCloux, Amy ; Woods, Amina S. ; Cotter, Robert J. ; Soloski, Mark J. ; Forman, James. / Identification of a tap-dependent leader peptide recognized by alloreactive T cells specific for a class Ib antigen. In: Cell. 1994 ; Vol. 79, No. 4. pp. 649-658.
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