Identification of acetylated proteins in Borrelia burgdorferi

Youyun Yang, Alan Wolfe, X. Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Posttranslational modification (PTM) of proteins has emerged as a major regulatory mechanism in all three domains of life. One emerging PTM is Nε-lysine acetylation—the acetylation of the epsilon amino group of lysine residues. Nε-lysine acetylation is known to regulate multiple cellular processes. In eukaryotes, it regulates chromatin structure, transcription, metabolism, signal transduction, and the cytoskeleton. Recently, multiple groups have detected Nε-lysine acetylation in diverse bacterial phyla, but no work on protein acetylation in Borrelia burgdorferi has been reported. Here, we describe a step-by-step protocol to identify Nε-lysine acetylated proteins in B. burgdorferi.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages177-182
Number of pages6
Volume1690
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1690
ISSN (Print)1064-3745

Fingerprint

Borrelia burgdorferi
Lysine
Acetylation
Post Translational Protein Processing
Proteins
Eukaryota
Cytoskeleton
Chromatin
Signal Transduction

Keywords

  • Acetylated lysine protein
  • Borrelia burgdorferi
  • Immunoprecipitation
  • Posttranslational modification
  • Protein acetylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yang, Y., Wolfe, A., & Yang, X. (2018). Identification of acetylated proteins in Borrelia burgdorferi. In Methods in Molecular Biology (Vol. 1690, pp. 177-182). (Methods in Molecular Biology; Vol. 1690). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7383-5_14

Identification of acetylated proteins in Borrelia burgdorferi. / Yang, Youyun; Wolfe, Alan; Yang, X.

Methods in Molecular Biology. Vol. 1690 Humana Press Inc., 2018. p. 177-182 (Methods in Molecular Biology; Vol. 1690).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yang, Y, Wolfe, A & Yang, X 2018, Identification of acetylated proteins in Borrelia burgdorferi. in Methods in Molecular Biology. vol. 1690, Methods in Molecular Biology, vol. 1690, Humana Press Inc., pp. 177-182. https://doi.org/10.1007/978-1-4939-7383-5_14
Yang Y, Wolfe A, Yang X. Identification of acetylated proteins in Borrelia burgdorferi. In Methods in Molecular Biology. Vol. 1690. Humana Press Inc. 2018. p. 177-182. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7383-5_14
Yang, Youyun ; Wolfe, Alan ; Yang, X. / Identification of acetylated proteins in Borrelia burgdorferi. Methods in Molecular Biology. Vol. 1690 Humana Press Inc., 2018. pp. 177-182 (Methods in Molecular Biology).
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