Identification of an α helical motif sufficient for association with papillomavirus E6

Jason J. Chen, Yihui Hong, Edward Rustamzadeh, James D. Baleja, Elliot Androphy

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

We recently identified a cellular protein named E6BP or ERC-55 that binds cancer-related papillomavirus E6 proteins (Chen, J. J., Reid, C. E., Band, V., and Androphy, E. J. (1995) Science 269, 529-531). By construction of a series of deletion mutants, the region of E6BP that is necessary and sufficient for complex formation with human papillomavirus type 16 E6 has been mapped to a 25-amino acid domain. The corresponding peptide was synthesized and found by nuclear magnetic resonance spectroscopy to bind calcium and fold into a classical helix-loop-helix EF-hand conformation. Additional deletion mutagenesis showed that 13 amino acids that form the second α helix mediated E6 association. Alanine replacement mutagenesis indicated that amino acids of this helix were most important for E6 binding. Alignment of this α helical E6 binding peptide with the 18-amino acid E6 binding region of E6AP (Hulbregtse, J. M., Scheffner, M., and Howley, P.M. (1993) Mol. Cell Biol. 13, 4918-4927) and the first LD repeat of another E6- binding protein, paxillin (Tong, X., and Howley, P.M. (1997) J. Biol. Chem. 272, 33373-33376), revealed substantial similarities among these E6 binding domains. The extent of homology and the mutational data define the peptide as an E6 binding motif.

Original languageEnglish (US)
Pages (from-to)13537-13544
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number22
DOIs
StatePublished - May 29 1998
Externally publishedYes

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Association reactions
Amino Acids
Mutagenesis
Peptides
Paxillin
EF Hand Motifs
Human papillomavirus 16
Alanine
Nuclear magnetic resonance spectroscopy
Conformations
Carrier Proteins
Proteins
Magnetic Resonance Spectroscopy
Calcium
Neoplasms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of an α helical motif sufficient for association with papillomavirus E6. / Chen, Jason J.; Hong, Yihui; Rustamzadeh, Edward; Baleja, James D.; Androphy, Elliot.

In: Journal of Biological Chemistry, Vol. 273, No. 22, 29.05.1998, p. 13537-13544.

Research output: Contribution to journalArticle

Chen, Jason J. ; Hong, Yihui ; Rustamzadeh, Edward ; Baleja, James D. ; Androphy, Elliot. / Identification of an α helical motif sufficient for association with papillomavirus E6. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 22. pp. 13537-13544.
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