Identification of B6SJL mSOD1G93A mouse subgroups with different disease progression rates

Melissa M. Haulcomb, Nichole A. Mesnard-Hoaglin, Richard J. Batka, Rena M. Meadows, Whitney M. Miller, Kathryn P. Mcmillan, Todd J. Brown, Virginia M. Sanders, Kathryn J. Jones

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Disease progression rates among patients with amyotrophic lateral sclerosis (ALS) vary greatly. Although the majority of affected individuals survive 3-5 years following diagnosis, some subgroups experience a more rapidly progressing form, surviving less than 1 year, and other subgroups experience slowly progressing forms, surviving nearly 50 years. Genetic heterogeneity and environmental factors pose significant barriers in investigating patient progression rates. Similar to the case for humans, variation in survival within the mSOD1 mouse has been well documented, but different progression rates have not been investigated. The present study identifies two subgroups of B6SJL mSOD1G93A mice with different disease progression rates, a fast progression group (FPG) and slow progression group, as evidenced by differences in the rate of motor function decline. In addition, increased disease-associated gene expression within the FPG facial motor nucleus confirmed the presence of a more severe phenotype. We hypothesize that a more severe disease phenotype could be the result of 1) an earlier onset of axonal disconnection with a consistent degeneration rate or 2) a more severe or accelerated degenerative process. We performed a facial nerve transection axotomy in both mSOD1 subgroups prior to disease onset as a method to standardize the axonal disconnection. Instead of leading to comparable gene expression in both subgroups, this standardization did not eliminate the severe phenotype in the FPG facial nucleus, suggesting that the FPG phenotype is the result of a more severe or accelerated degenerative process. We theorize that these mSOD1 subgroups are representative of the rapid and slow disease phenotypes often experienced in ALS.

Original languageEnglish (US)
Pages (from-to)2752-2768
Number of pages17
JournalJournal of Comparative Neurology
Volume523
Issue number18
DOIs
StatePublished - Dec 15 2015

Keywords

  • ALS
  • Disease progression
  • Facial nerve axotomy
  • Gene expression
  • MN
  • MSOD1
  • Motoneuron

ASJC Scopus subject areas

  • Neuroscience(all)

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    Haulcomb, M. M., Mesnard-Hoaglin, N. A., Batka, R. J., Meadows, R. M., Miller, W. M., Mcmillan, K. P., Brown, T. J., Sanders, V. M., & Jones, K. J. (2015). Identification of B6SJL mSOD1G93A mouse subgroups with different disease progression rates. Journal of Comparative Neurology, 523(18), 2752-2768. https://doi.org/10.1002/cne.23814