Identification of Caenorhabditis elegans genes required for neuronal differentiation and migration

Wayne Forrester, Elliot Perens, Jennifer A. Zallen, Gian Garriga

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

To understand the mechanisms that guide migrating cells, we have been studying the embryonic migrations of the C. elegans canal-associated neurons (CANs). Here, we describe two screens used to identify genes involved in CAN migration. First, we screened for mutants that died as clear larvae (Clr) or had withered tails (Wit), phenotypes displayed by animals lacking normal CAN function. Second, we screened directly for mutants with missing or misplaced CANs. We isolated and characterized 30 mutants that defined 14 genes necessary for CAN migration. We found that one of the genes, ceh-10, specifies CAN fate. ceh-10 had been defined molecularly as encoding a homeodomain protein expressed in the CANs. Mutations that reduce ceh-10 function result in Wit animals with CANs that are partially defective in their migrations. Mutations that eliminate ceh-10 function result in Clr animals with CANs that fail to migrate or express CEH-23, a CAN differentiation marker. Null mutants also fail to express CEH-10, suggesting that CEH-10 regulates its own expression. Finally, we found that ceh-10 is necessary for the differentiation of AIY and RMED, two additional cells that express CEH-10.

Original languageEnglish (US)
Pages (from-to)151-165
Number of pages15
JournalGenetics
Volume148
Issue number1
StatePublished - Jan 1998
Externally publishedYes

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Caenorhabditis elegans
Neurons
Genes
Larva
Tail
Homeodomain Proteins
Mutation
Differentiation Antigens
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Identification of Caenorhabditis elegans genes required for neuronal differentiation and migration. / Forrester, Wayne; Perens, Elliot; Zallen, Jennifer A.; Garriga, Gian.

In: Genetics, Vol. 148, No. 1, 01.1998, p. 151-165.

Research output: Contribution to journalArticle

Forrester, Wayne ; Perens, Elliot ; Zallen, Jennifer A. ; Garriga, Gian. / Identification of Caenorhabditis elegans genes required for neuronal differentiation and migration. In: Genetics. 1998 ; Vol. 148, No. 1. pp. 151-165.
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