Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation

Midori Iida, Toshio Heike, Momoko Yoshimoto, Shiro Baba, Hiraku Doi, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We evaluated the expression of the FLK1, one of the lateral mesoderm early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1+ cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1- cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1+ cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1+ CD31-VE-cadherin- phenotype. Our findings indicate that FLK1+ cells, especially FLK1+ CD31-VE-cadherin- cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalFASEB Journal
Volume19
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

cadherins
Endothelial cells
embryonic stem cells
Embryonic Stem Cells
Stem cells
cell differentiation
stem cells
Cell Differentiation
Stem Cells
cells
Embryoid Bodies
Endothelial Cells
endothelial cells
cadherin 5
Mesoderm
Stromal Cells
Coculture Techniques
stromal cells
coculture
Phenotype

Keywords

  • Blastocysts
  • Cardiomyocyte differentiation
  • Embryoid bodies
  • ES cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation. / Iida, Midori; Heike, Toshio; Yoshimoto, Momoko; Baba, Shiro; Doi, Hiraku; Nakahata, Tatsutoshi.

In: FASEB Journal, Vol. 19, No. 3, 03.2005, p. 371-378.

Research output: Contribution to journalArticle

Iida, Midori ; Heike, Toshio ; Yoshimoto, Momoko ; Baba, Shiro ; Doi, Hiraku ; Nakahata, Tatsutoshi. / Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation. In: FASEB Journal. 2005 ; Vol. 19, No. 3. pp. 371-378.
@article{74a8e333fc2b4e88b7e895a744fdfefa,
title = "Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation",
abstract = "We evaluated the expression of the FLK1, one of the lateral mesoderm early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1+ cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1- cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1+ cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1+ CD31-VE-cadherin- phenotype. Our findings indicate that FLK1+ cells, especially FLK1+ CD31-VE-cadherin- cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.",
keywords = "Blastocysts, Cardiomyocyte differentiation, Embryoid bodies, ES cells",
author = "Midori Iida and Toshio Heike and Momoko Yoshimoto and Shiro Baba and Hiraku Doi and Tatsutoshi Nakahata",
year = "2005",
month = "3",
doi = "10.1096/fj.04-1998com",
language = "English (US)",
volume = "19",
pages = "371--378",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation

AU - Iida, Midori

AU - Heike, Toshio

AU - Yoshimoto, Momoko

AU - Baba, Shiro

AU - Doi, Hiraku

AU - Nakahata, Tatsutoshi

PY - 2005/3

Y1 - 2005/3

N2 - We evaluated the expression of the FLK1, one of the lateral mesoderm early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1+ cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1- cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1+ cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1+ CD31-VE-cadherin- phenotype. Our findings indicate that FLK1+ cells, especially FLK1+ CD31-VE-cadherin- cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.

AB - We evaluated the expression of the FLK1, one of the lateral mesoderm early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1+ cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1- cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1+ cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1+ CD31-VE-cadherin- phenotype. Our findings indicate that FLK1+ cells, especially FLK1+ CD31-VE-cadherin- cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.

KW - Blastocysts

KW - Cardiomyocyte differentiation

KW - Embryoid bodies

KW - ES cells

UR - http://www.scopus.com/inward/record.url?scp=14644441633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644441633&partnerID=8YFLogxK

U2 - 10.1096/fj.04-1998com

DO - 10.1096/fj.04-1998com

M3 - Article

VL - 19

SP - 371

EP - 378

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -