Identification of cell surface receptors for murine macrophage inflammatory protein-1α

K. O. Oh, Z. Zhou, K. K. Kim, H. Samanta, M. Fraser, Y. J. Kim, H. E. Broxmeyer, B. S. Kwon

Research output: Contribution to journalArticle

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Abstract

We have produced recombinant proteins for a cytokine, L2G25BP (macrophage inflammatory protein-1α) (MIP-1α). By using the recombinant protein (rMIP-1α), receptors for MIP-1α were identified on Con A-stimulated and unstimulated CTLL-R8, a T cell line, and LPS-stimulated RAW 264.7, a macrophage cell line. The 125I-rMIP-1α binds to the receptor in a specific and saturable manner. Scatchard analysis indicated a single class of high affinity receptor, with a Kd of approximately 1.5 × 10-9 M and approximately 1200 binding sites/Con A-stimulated CTLL-R8 cell and a Kd of 0.9 × 10-9 M and approximately 380 binding sites/RAW 264.7 cell. 125I-rMIP-1α binding was inhibited by unlabeled rMIP-1α in a dose-dependent manner, but not by IL-1α or IL-2. rMIP-1α inhibited the proliferation of unstimulated CTLL-R8 cells. Rabbit anti-rMIP-1α antibodies blocked the growth-inhibitory effect of the rMIP-1α on CTLL-R8 cells.

Original languageEnglish (US)
Pages (from-to)2978-2983
Number of pages6
JournalJournal of Immunology
Volume147
Issue number9
StatePublished - Jan 1 1991

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Macrophage Inflammatory Proteins
Cell Surface Receptors
Recombinant Proteins
Binding Sites
Cell Line
Interleukin-1
Interleukin-2
Macrophages
Cytokines
Rabbits
T-Lymphocytes
Antibodies
Growth

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Oh, K. O., Zhou, Z., Kim, K. K., Samanta, H., Fraser, M., Kim, Y. J., ... Kwon, B. S. (1991). Identification of cell surface receptors for murine macrophage inflammatory protein-1α. Journal of Immunology, 147(9), 2978-2983.

Identification of cell surface receptors for murine macrophage inflammatory protein-1α. / Oh, K. O.; Zhou, Z.; Kim, K. K.; Samanta, H.; Fraser, M.; Kim, Y. J.; Broxmeyer, H. E.; Kwon, B. S.

In: Journal of Immunology, Vol. 147, No. 9, 01.01.1991, p. 2978-2983.

Research output: Contribution to journalArticle

Oh, KO, Zhou, Z, Kim, KK, Samanta, H, Fraser, M, Kim, YJ, Broxmeyer, HE & Kwon, BS 1991, 'Identification of cell surface receptors for murine macrophage inflammatory protein-1α', Journal of Immunology, vol. 147, no. 9, pp. 2978-2983.
Oh KO, Zhou Z, Kim KK, Samanta H, Fraser M, Kim YJ et al. Identification of cell surface receptors for murine macrophage inflammatory protein-1α. Journal of Immunology. 1991 Jan 1;147(9):2978-2983.
Oh, K. O. ; Zhou, Z. ; Kim, K. K. ; Samanta, H. ; Fraser, M. ; Kim, Y. J. ; Broxmeyer, H. E. ; Kwon, B. S. / Identification of cell surface receptors for murine macrophage inflammatory protein-1α. In: Journal of Immunology. 1991 ; Vol. 147, No. 9. pp. 2978-2983.
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