We have produced recombinant proteins for a cytokine, L2G25BP (macrophage inflammatory protein-1α) (MIP-1α). By using the recombinant protein (rMIP-1α), receptors for MIP-1α were identified on Con A-stimulated and unstimulated CTLL-R8, a T cell line, and LPS-stimulated RAW 264.7, a macrophage cell line. The 125I-rMIP-1α binds to the receptor in a specific and saturable manner. Scatchard analysis indicated a single class of high affinity receptor, with a Kd of approximately 1.5 × 10-9 M and approximately 1200 binding sites/Con A-stimulated CTLL-R8 cell and a Kd of 0.9 × 10-9 M and approximately 380 binding sites/RAW 264.7 cell. 125I-rMIP-1α binding was inhibited by unlabeled rMIP-1α in a dose-dependent manner, but not by IL-1α or IL-2. rMIP-1α inhibited the proliferation of unstimulated CTLL-R8 cells. Rabbit anti-rMIP-1α antibodies blocked the growth-inhibitory effect of the rMIP-1α on CTLL-R8 cells.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - 1991|
ASJC Scopus subject areas
- Immunology and Allergy