Identification of cell surface receptors for murine macrophage inflammatory protein-1α

K. O. Oh, Z. Zhou, K. K. Kim, H. Samanta, M. Fraser, Y. J. Kim, H. E. Broxmeyer, B. S. Kwon

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Abstract

We have produced recombinant proteins for a cytokine, L2G25BP (macrophage inflammatory protein-1α) (MIP-1α). By using the recombinant protein (rMIP-1α), receptors for MIP-1α were identified on Con A-stimulated and unstimulated CTLL-R8, a T cell line, and LPS-stimulated RAW 264.7, a macrophage cell line. The 125I-rMIP-1α binds to the receptor in a specific and saturable manner. Scatchard analysis indicated a single class of high affinity receptor, with a Kd of approximately 1.5 × 10-9 M and approximately 1200 binding sites/Con A-stimulated CTLL-R8 cell and a Kd of 0.9 × 10-9 M and approximately 380 binding sites/RAW 264.7 cell. 125I-rMIP-1α binding was inhibited by unlabeled rMIP-1α in a dose-dependent manner, but not by IL-1α or IL-2. rMIP-1α inhibited the proliferation of unstimulated CTLL-R8 cells. Rabbit anti-rMIP-1α antibodies blocked the growth-inhibitory effect of the rMIP-1α on CTLL-R8 cells.

Original languageEnglish (US)
Pages (from-to)2978-2983
Number of pages6
JournalJournal of Immunology
Volume147
Issue number9
StatePublished - Jan 1 1991

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Oh, K. O., Zhou, Z., Kim, K. K., Samanta, H., Fraser, M., Kim, Y. J., Broxmeyer, H. E., & Kwon, B. S. (1991). Identification of cell surface receptors for murine macrophage inflammatory protein-1α. Journal of Immunology, 147(9), 2978-2983.