Identification of cell thermal microenvironment in porcine skin during laser cauterization

Sagar Bhogle, Gayatriben Mistri, Benjamin Vesper, Steven Lacey, James Radosevich, Michael Colvard, Elisa Budyn

Research output: Contribution to journalArticle

Abstract

In order to minimize bleeding during surgical intervention, laser devices make it possible to produce local heat for cauterization and to reduce the blood loss at the site of the incision in the skin. However, the method is sometimes associated with local overheating and tissue partial vaporization. We present a 3D Finite Element Model (FEM) hierarchical model to simulate the effect of heat produced by different surgical laser devices on the skin. The model includes the macro-molecular, cell and tissue scales. The model follows the bio-heat equation where the transient heat diffusion includes heat conduction in the tissue, convection with the surrounding air, the metabolic heat and the heat source of the laser device. The laser heat flow propagating in the skin was assumed to exponentially decrease from the opening edge of the wound. A set of different lasers were tested on live porcine skin under general anesthesia. The skin morphology of harvested samples along the wound was digitally surveyed in light microscopy observations of the skin specimens that had been previously stained with an anti-swine antibody. The biological constituents of the skin tissue were modeled as separate continua of specific thermal properties taken from the literature in the presented preliminary model. The numerical model evaluated the temperature and the heat flux fields in the vicinity of the laser wound in porcine skin. Using an inverse procedure, these results were validated by comparison with antibody stained tissue images of the experimental samples in order to identify the temperature at which significant damage is experienced by skin cells.

Original languageEnglish (US)
Pages (from-to)19-45
Number of pages27
JournalComputer Methods in Applied Mechanics and Engineering
Volume314
DOIs
StatePublished - Feb 1 2017

Fingerprint

Skin
Lasers
cells
lasers
heat
Tissue
antibodies
anesthesia
Antibodies
bleeding
swine
heat sources
heat transmission
conductive heat transfer
blood
Hot Temperature
heat flux
convection
thermodynamic properties
continuums

Keywords

  • Cauterization
  • Laser
  • Skin
  • Thermal property

ASJC Scopus subject areas

  • Computational Mechanics
  • Mechanics of Materials
  • Mechanical Engineering
  • Physics and Astronomy(all)
  • Computer Science Applications

Cite this

Identification of cell thermal microenvironment in porcine skin during laser cauterization. / Bhogle, Sagar; Mistri, Gayatriben; Vesper, Benjamin; Lacey, Steven; Radosevich, James; Colvard, Michael; Budyn, Elisa.

In: Computer Methods in Applied Mechanics and Engineering, Vol. 314, 01.02.2017, p. 19-45.

Research output: Contribution to journalArticle

Bhogle, Sagar ; Mistri, Gayatriben ; Vesper, Benjamin ; Lacey, Steven ; Radosevich, James ; Colvard, Michael ; Budyn, Elisa. / Identification of cell thermal microenvironment in porcine skin during laser cauterization. In: Computer Methods in Applied Mechanics and Engineering. 2017 ; Vol. 314. pp. 19-45.
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