Identification of committed mononuclear precursors for osteoclast-like cells formed in long term human marrow cultures

Noriyoshi Kurihara, C. Chenu, M. Miller, C. Civin, G. David Roodman

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor cells were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor for 9 days to form colony forming unitgranulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of the colonies that formed were composed of cells with a unique polygonal morphology. One hundred percent of the polygonal cells in these colonies cross-reacted with the monoclonal antibody 23c6, which preferentially recognizes osteoclasts. Homogenous populations of these polygonal cells formed multinucleated cells (MNC) in suspension culture, 100% of which cross-reacted with the 23c6 monoclonal antibody, and greater than 90% of the MNC contracted in response to calcitonin. Approximately 20% of these MNC formed resorption lacunae on calcified matrices. These results suggest that 1) early osteoclast precursors are derived from CFU-GM, the committed granulocyte-macrophage progenitor; 2) committed mononuclear osteoclast precursors have a distinct polygonal morphology and cross-react with monoclonal antibodies that recognize mature osteoclasts; and 3) these mononuclear precursors are capable of forming multinucleated cells which fulfill the functional criteria for osteoclasts.

Original languageEnglish (US)
Pages (from-to)2733-2741
Number of pages9
JournalEndocrinology
Volume126
Issue number5
StatePublished - May 1990
Externally publishedYes

Fingerprint

Osteoclasts
Bone Marrow
Monoclonal Antibodies
Macrophages
Granulocyte-Macrophage Progenitor Cells
Calcitonin
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoietic Stem Cells
Suspensions
Population

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Identification of committed mononuclear precursors for osteoclast-like cells formed in long term human marrow cultures. / Kurihara, Noriyoshi; Chenu, C.; Miller, M.; Civin, C.; Roodman, G. David.

In: Endocrinology, Vol. 126, No. 5, 05.1990, p. 2733-2741.

Research output: Contribution to journalArticle

@article{2ecf5c80dc5b4082abb40c0b2492c01f,
title = "Identification of committed mononuclear precursors for osteoclast-like cells formed in long term human marrow cultures",
abstract = "Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor cells were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor for 9 days to form colony forming unitgranulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30{\%} of the colonies that formed were composed of cells with a unique polygonal morphology. One hundred percent of the polygonal cells in these colonies cross-reacted with the monoclonal antibody 23c6, which preferentially recognizes osteoclasts. Homogenous populations of these polygonal cells formed multinucleated cells (MNC) in suspension culture, 100{\%} of which cross-reacted with the 23c6 monoclonal antibody, and greater than 90{\%} of the MNC contracted in response to calcitonin. Approximately 20{\%} of these MNC formed resorption lacunae on calcified matrices. These results suggest that 1) early osteoclast precursors are derived from CFU-GM, the committed granulocyte-macrophage progenitor; 2) committed mononuclear osteoclast precursors have a distinct polygonal morphology and cross-react with monoclonal antibodies that recognize mature osteoclasts; and 3) these mononuclear precursors are capable of forming multinucleated cells which fulfill the functional criteria for osteoclasts.",
author = "Noriyoshi Kurihara and C. Chenu and M. Miller and C. Civin and Roodman, {G. David}",
year = "1990",
month = "5",
language = "English (US)",
volume = "126",
pages = "2733--2741",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "5",

}

TY - JOUR

T1 - Identification of committed mononuclear precursors for osteoclast-like cells formed in long term human marrow cultures

AU - Kurihara, Noriyoshi

AU - Chenu, C.

AU - Miller, M.

AU - Civin, C.

AU - Roodman, G. David

PY - 1990/5

Y1 - 1990/5

N2 - Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor cells were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor for 9 days to form colony forming unitgranulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of the colonies that formed were composed of cells with a unique polygonal morphology. One hundred percent of the polygonal cells in these colonies cross-reacted with the monoclonal antibody 23c6, which preferentially recognizes osteoclasts. Homogenous populations of these polygonal cells formed multinucleated cells (MNC) in suspension culture, 100% of which cross-reacted with the 23c6 monoclonal antibody, and greater than 90% of the MNC contracted in response to calcitonin. Approximately 20% of these MNC formed resorption lacunae on calcified matrices. These results suggest that 1) early osteoclast precursors are derived from CFU-GM, the committed granulocyte-macrophage progenitor; 2) committed mononuclear osteoclast precursors have a distinct polygonal morphology and cross-react with monoclonal antibodies that recognize mature osteoclasts; and 3) these mononuclear precursors are capable of forming multinucleated cells which fulfill the functional criteria for osteoclasts.

AB - Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor cells were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor for 9 days to form colony forming unitgranulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of the colonies that formed were composed of cells with a unique polygonal morphology. One hundred percent of the polygonal cells in these colonies cross-reacted with the monoclonal antibody 23c6, which preferentially recognizes osteoclasts. Homogenous populations of these polygonal cells formed multinucleated cells (MNC) in suspension culture, 100% of which cross-reacted with the 23c6 monoclonal antibody, and greater than 90% of the MNC contracted in response to calcitonin. Approximately 20% of these MNC formed resorption lacunae on calcified matrices. These results suggest that 1) early osteoclast precursors are derived from CFU-GM, the committed granulocyte-macrophage progenitor; 2) committed mononuclear osteoclast precursors have a distinct polygonal morphology and cross-react with monoclonal antibodies that recognize mature osteoclasts; and 3) these mononuclear precursors are capable of forming multinucleated cells which fulfill the functional criteria for osteoclasts.

UR - http://www.scopus.com/inward/record.url?scp=0025294673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025294673&partnerID=8YFLogxK

M3 - Article

VL - 126

SP - 2733

EP - 2741

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -