Identification of Elongin C and Skp1 sequences that determine cullin selection

Qin Yan, Takumi Kamura, Yong Cai, Jingji Jin, Mircea Ivan, Arcady Mushegian, Ronald C. Conaway, Joan Weliky Conaway

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The multiprotein von Hippel-Lindau (VHL) tumor suppressor and Skp1-Cul1-F-box protein (SCF) complexes belong to families of structurally related E3 ubiquitin ligases. In the VHL ubiquitin ligase, the VHL protein serves as the substrate recognition subunit, which is linked by the adaptor protein Elongin C to a heterodimeric Cul2/Rbx1 module that activates ubiquitylation of target proteins by the E2 ubiquitin-conjugating enzyme Ubc5. In SCF ubiquitin ligases, F-box proteins serve as substrate recognition subunits, which are linked by the Elongin C-like adaptor protein Skp1 to a Cul1/Rbx1 module that activates ubiquitylation of target proteins, in most cases by the E2 Cdc34. In this report, we investigate the functions of the Elongin C and Skp1 proteins in reconstitution of VBDL and SCF ubiquitin ligases. We identify Elongin C and Skp1 structural elements responsible for selective interaction with their cognate Cullin/Hbx1 modules. In addition, using altered specificity Elongin C and F-box protein mutants, we investigate models for the mechanism underlying E2 selection by VHL and SCF ubiquitin ligases. Our findings provide evidence that E2 selection by VHL and SCF ubiquitin ligases is determined not solely by the Cullin/ Rbx1 module, the target protein, or the integrity of the substrate recognition subunit but by yet to be elucidated features of these macromolecular complexes.

Original languageEnglish (US)
Pages (from-to)43019-43026
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number41
DOIs
StatePublished - Oct 8 2004
Externally publishedYes

Fingerprint

Cullin Proteins
F-Box Proteins
Ligases
Ubiquitin
S-Phase Kinase-Associated Proteins
Ubiquitination
Proteins
Substrates
Ubiquitin-Conjugating Enzymes
Macromolecular Substances
Ubiquitin-Protein Ligases
Protein C
elongin
Tumors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yan, Q., Kamura, T., Cai, Y., Jin, J., Ivan, M., Mushegian, A., ... Conaway, J. W. (2004). Identification of Elongin C and Skp1 sequences that determine cullin selection. Journal of Biological Chemistry, 279(41), 43019-43026. https://doi.org/10.1074/jbc.M408018200

Identification of Elongin C and Skp1 sequences that determine cullin selection. / Yan, Qin; Kamura, Takumi; Cai, Yong; Jin, Jingji; Ivan, Mircea; Mushegian, Arcady; Conaway, Ronald C.; Conaway, Joan Weliky.

In: Journal of Biological Chemistry, Vol. 279, No. 41, 08.10.2004, p. 43019-43026.

Research output: Contribution to journalArticle

Yan, Q, Kamura, T, Cai, Y, Jin, J, Ivan, M, Mushegian, A, Conaway, RC & Conaway, JW 2004, 'Identification of Elongin C and Skp1 sequences that determine cullin selection', Journal of Biological Chemistry, vol. 279, no. 41, pp. 43019-43026. https://doi.org/10.1074/jbc.M408018200
Yan, Qin ; Kamura, Takumi ; Cai, Yong ; Jin, Jingji ; Ivan, Mircea ; Mushegian, Arcady ; Conaway, Ronald C. ; Conaway, Joan Weliky. / Identification of Elongin C and Skp1 sequences that determine cullin selection. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 41. pp. 43019-43026.
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