Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas

L. Banks, P. Spence, E. Androphy, N. Hubbert, G. Matlashewski, A. Murray, L. Crawford

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6 protein between HPV-16 and HPV-18, with only one antibody recognizing a cross-reactive epitope. We have used both the monoclonal and the polyclonal antibodies to look for E6 expression in a number of HPV DNA-containing cell lines. These reagents specifically detected a 16.5K mol. wt. polypeptide in cells derived from a human cervical carcinoma.

Original languageEnglish (US)
Pages (from-to)1351-1359
Number of pages9
JournalJournal of General Virology
Volume68
Issue number5
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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