Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas

L. Banks, P. Spence, Elliot Androphy, N. Hubbert, G. Matlashewski, A. Murray, L. Crawford

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6 protein between HPV-16 and HPV-18, with only one antibody recognizing a cross-reactive epitope. We have used both the monoclonal and the polyclonal antibodies to look for E6 expression in a number of HPV DNA-containing cell lines. These reagents specifically detected a 16.5K mol. wt. polypeptide in cells derived from a human cervical carcinoma.

Original languageEnglish (US)
Pages (from-to)1351-1359
Number of pages9
JournalJournal of General Virology
Volume68
Issue number5
StatePublished - 1987
Externally publishedYes

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Human papillomavirus 18
Carcinoma
Peptides
Antibodies
Monoclonal Antibodies
Epitopes
Bacteria
Cell Line
DNA
Human papillomavirus type 18 E6 protein

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Banks, L., Spence, P., Androphy, E., Hubbert, N., Matlashewski, G., Murray, A., & Crawford, L. (1987). Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas. Journal of General Virology, 68(5), 1351-1359.

Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas. / Banks, L.; Spence, P.; Androphy, Elliot; Hubbert, N.; Matlashewski, G.; Murray, A.; Crawford, L.

In: Journal of General Virology, Vol. 68, No. 5, 1987, p. 1351-1359.

Research output: Contribution to journalArticle

Banks, L, Spence, P, Androphy, E, Hubbert, N, Matlashewski, G, Murray, A & Crawford, L 1987, 'Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas', Journal of General Virology, vol. 68, no. 5, pp. 1351-1359.
Banks, L. ; Spence, P. ; Androphy, Elliot ; Hubbert, N. ; Matlashewski, G. ; Murray, A. ; Crawford, L. / Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas. In: Journal of General Virology. 1987 ; Vol. 68, No. 5. pp. 1351-1359.
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