Identification of link proteins in canine synovial cell cultures and canine articular cartilage

Rose Fife, B. Caterson, S. L. Myers

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Link proteins are glycoproteins in cartilage that are involved in the stabilization of aggregates of proteoglycans and hyaluronic acid. We have identified link proteins in synovial cell cultures from normal canine synovium using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunofluorescence, and immunolocation with specific antibodies by electrophoretic transfer. We have also found evidence for the synthesis of link proteins in these cultures by fluorography of radiolabeled synovial cell extracts. We have identified a 70,000-mol-wt protein in canine synovial cell culture extracts that has antigenic cross-reactivity with the 48,000-mol-wt link protein. Three link proteins were identified in normal canine articular cartilage. These results indicate that link proteins are more widely distributed in connective tissues than previously recognized and may have biological functions other than aggregate stabilization.

Original languageEnglish
Pages (from-to)1050-1055
Number of pages6
JournalJournal of Cell Biology
Volume100
Issue number4
StatePublished - 1985

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Articular Cartilage
Canidae
Cell Culture Techniques
Cell Extracts
Photofluorography
Synovial Membrane
Proteoglycans
Hyaluronic Acid
Sodium Dodecyl Sulfate
Connective Tissue
Cartilage
Fluorescent Antibody Technique
link protein
Polyacrylamide Gel Electrophoresis
Glycoproteins
Antibodies
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Identification of link proteins in canine synovial cell cultures and canine articular cartilage. / Fife, Rose; Caterson, B.; Myers, S. L.

In: Journal of Cell Biology, Vol. 100, No. 4, 1985, p. 1050-1055.

Research output: Contribution to journalArticle

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