Identification of markers for nipple epidermis: Changes in expression during pregnancy and lactation

Jennifer Eastwood, Carlos Offutt, Keshav Menon, Mitchell Keel, Petra Hrncirova, Milos V. Novotny, Randy Arnold, John Foley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In vertebrates, specific regions of skin crucial for interaction with and manipulation of elements in the environment are characterized by specialized epidermis. Regions of specialized epidermis show distinct patterns of cellular differentiation and express specific keratins that provide an increased ability to withstand mechanical strain. The nipple, which must endure the mechanical strain of nursing, is a type of specialized epidermis. The entire ventral skin of the keratin 14 promoter driven PTHrP mouse provides a model for nipple development. To identify novel markers for this specialized epidermis, we have used two-dimensional (2-D) gels, mass spectrometric protein identification, Western blotting and immunohistochemistry to compare intermediate filament preparations from the nipple-like K14-PTHrP ventral skin to that of wild-type littermates. We identified 64 spots on 2-D gels that were increased in expression in the nipple-like skin of the female K14-PTHrP mouse and 11 spots that were elevated in the wild type. Microsequencing suggested that K17 and epiplakin were among the proteins with the greatest increase in expression in the K14-PTHrP ventral skin. Using Western blots and immunohistochemistry, we evaluated the expression of these proteins as well as K6 in the wild-type nipple, K14-PTHrP ventral skin and wild-type ventral skin. In addition, we found that the expression of K6 was minimally changed in the pregnant and lactating nipple, but the expression of a previously identified marker, K2e, was reduced during lactation. Using a model of the mechanical strain induced by nursing, we found that K2e but not K6 expression was responsive to this condition. The identification of epidermal markers and their expression patterns will provide insight into the cellular differentiation patterns of the nipple and the underlying epidermal-mesenchymal interactions that direct this differentiation.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalDifferentiation
Volume75
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Nipples
Lactation
Epidermis
Parathyroid Hormone-Related Protein
Pregnancy
Skin
Nursing
Western Blotting
Gels
Immunohistochemistry
Keratin-14
Proteins
Intermediate Filaments
Keratins
Vertebrates

Keywords

  • Epiplakin
  • Keratins
  • Lactation
  • Nipple
  • PTHrP

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Identification of markers for nipple epidermis : Changes in expression during pregnancy and lactation. / Eastwood, Jennifer; Offutt, Carlos; Menon, Keshav; Keel, Mitchell; Hrncirova, Petra; Novotny, Milos V.; Arnold, Randy; Foley, John.

In: Differentiation, Vol. 75, No. 1, 01.2007, p. 75-83.

Research output: Contribution to journalArticle

Eastwood, J, Offutt, C, Menon, K, Keel, M, Hrncirova, P, Novotny, MV, Arnold, R & Foley, J 2007, 'Identification of markers for nipple epidermis: Changes in expression during pregnancy and lactation', Differentiation, vol. 75, no. 1, pp. 75-83. https://doi.org/10.1111/j.1432-0436.2006.00112.x
Eastwood, Jennifer ; Offutt, Carlos ; Menon, Keshav ; Keel, Mitchell ; Hrncirova, Petra ; Novotny, Milos V. ; Arnold, Randy ; Foley, John. / Identification of markers for nipple epidermis : Changes in expression during pregnancy and lactation. In: Differentiation. 2007 ; Vol. 75, No. 1. pp. 75-83.
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