Identification of Metabolic Changes in Ileum, Jejunum, Skeletal Muscle, Liver, and Lung in a Continuous I.V. Pseudomonas aeruginosa Model of Sepsis Using Nontargeted Metabolomics Analysis

Amro Ilaiwy, Gabriella A.M. ten Have, James R. Bain, Michael J. Muehlbauer, Sara K. O'Neal, Jessica M. Berthiaume, Traci L. Parry, Nicolaas E. Deutz, Monte Willis

Research output: Contribution to journalArticle

Abstract

Sepsis is a multiorgan disease affecting the ileum and jejunum (small intestine), liver, skeletal muscle, and lung clinically. The specific metabolic changes in the ileum, jejunum, liver, skeletal muscle, and lung have not previously been investigated. Live Pseudomonas aeruginosa, isolated from a patient, was given via i.v. catheter to pigs to induce severe sepsis. Eighteen hours later, ileum, jejunum, medial gastrocnemius skeletal muscle, liver, and lung were analyzed by nontargeted metabolomics analysis using gas chromatography/mass spectrometry. The ileum and the liver demonstrated significant changes in metabolites involved in linoleic acid metabolism: the ileum and lung had significant changes in the metabolism of valine/leucine/isoleucine; the jejunum, skeletal muscle, and liver had significant changes in arginine/proline metabolism; and the skeletal muscle and lung had significant changes in aminoacyl-tRNA biosynthesis, as analyzed by pathway analysis. Pathway analysis also identified changes in metabolic pathways unique for different tissues, including changes in the citric acid cycle (jejunum), β-alanine metabolism (skeletal muscle), and purine metabolism (liver). These findings demonstrate both overlapping metabolic pathways affected in different tissues and those that are unique to others and provide insight into the metabolic changes in sepsis leading to organ dysfunction. This may allow therapeutic interventions that focus on multiple tissues or single tissues once the relationship of the altered metabolites/metabolism to the underlying pathogenesis of sepsis is determined.

Original languageEnglish (US)
Pages (from-to)1797-1813
Number of pages17
JournalAmerican Journal of Pathology
Volume189
Issue number9
DOIs
StatePublished - Sep 1 2019

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Metabolomics
Jejunum
Ileum
Pseudomonas aeruginosa
Sepsis
Skeletal Muscle
Lung
Liver
Metabolic Networks and Pathways
Citric Acid Cycle
Isoleucine
Valine
Linoleic Acid
Transfer RNA
Proline
Leucine
Alanine
Gas Chromatography-Mass Spectrometry
Small Intestine
Arginine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Identification of Metabolic Changes in Ileum, Jejunum, Skeletal Muscle, Liver, and Lung in a Continuous I.V. Pseudomonas aeruginosa Model of Sepsis Using Nontargeted Metabolomics Analysis. / Ilaiwy, Amro; ten Have, Gabriella A.M.; Bain, James R.; Muehlbauer, Michael J.; O'Neal, Sara K.; Berthiaume, Jessica M.; Parry, Traci L.; Deutz, Nicolaas E.; Willis, Monte.

In: American Journal of Pathology, Vol. 189, No. 9, 01.09.2019, p. 1797-1813.

Research output: Contribution to journalArticle

Ilaiwy, Amro ; ten Have, Gabriella A.M. ; Bain, James R. ; Muehlbauer, Michael J. ; O'Neal, Sara K. ; Berthiaume, Jessica M. ; Parry, Traci L. ; Deutz, Nicolaas E. ; Willis, Monte. / Identification of Metabolic Changes in Ileum, Jejunum, Skeletal Muscle, Liver, and Lung in a Continuous I.V. Pseudomonas aeruginosa Model of Sepsis Using Nontargeted Metabolomics Analysis. In: American Journal of Pathology. 2019 ; Vol. 189, No. 9. pp. 1797-1813.
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AU - ten Have, Gabriella A.M.

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AU - O'Neal, Sara K.

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