Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two‐color fluorescence in situ hybridization (FISH)

Teresa A. Smolarek, Ruthann I. Blough, Richard S. Foster, Thomas M. Ulbright, Catherine G. Palmer, Nyla A. Heerema

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27 Citations (Scopus)

Abstract

The distribution of segments of the short and long arms of chromosome 12 was distinguished by two‐color fluorescence in situ hybridization (FISH) in 27 cytogenetically abnormal testicular germ cell tumors (TGCTs). A 12p‐specific probe was developed by chromosomal microdissection and sequence‐independent polymerase chain reaction (PCR) amplification and was combined with a commercially available whole‐chromosome 12 painting probe. The TGCTs included both i(12p)‐positive and i(12p)‐negative primary tumors and lymph node metastases from patients in clinical stage I or stage II who were not previously treated with chemotherapy. Rearrangements of the short arm of chromosome 12 and overrepresentation of 12p DNA sequences were found in all cases. In addition, cryptic rearrangements of 12p were found in 39% (7/18) of the i(12p)‐positive tumors and in 78% (7/9) of the i(12p)‐negative tumors. Only 7% (2/27) of all tumors had cryptic rearrangements of 12q.

Original languageEnglish (US)
Pages (from-to)252-258
Number of pages7
JournalGenes, Chromosomes and Cancer
Volume14
Issue number4
DOIs
StatePublished - Dec 1995

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Chromosomes, Human, Pair 12
Fluorescence In Situ Hybridization
Chromosome Aberrations
Color
Neoplasms
Chromosome Painting
Microdissection
Lymph Nodes
Neoplasm Metastasis
Drug Therapy
Polymerase Chain Reaction
Testicular Germ Cell Tumor

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two‐color fluorescence in situ hybridization (FISH). / Smolarek, Teresa A.; Blough, Ruthann I.; Foster, Richard S.; Ulbright, Thomas M.; Palmer, Catherine G.; Heerema, Nyla A.

In: Genes, Chromosomes and Cancer, Vol. 14, No. 4, 12.1995, p. 252-258.

Research output: Contribution to journalArticle

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abstract = "The distribution of segments of the short and long arms of chromosome 12 was distinguished by two‐color fluorescence in situ hybridization (FISH) in 27 cytogenetically abnormal testicular germ cell tumors (TGCTs). A 12p‐specific probe was developed by chromosomal microdissection and sequence‐independent polymerase chain reaction (PCR) amplification and was combined with a commercially available whole‐chromosome 12 painting probe. The TGCTs included both i(12p)‐positive and i(12p)‐negative primary tumors and lymph node metastases from patients in clinical stage I or stage II who were not previously treated with chemotherapy. Rearrangements of the short arm of chromosome 12 and overrepresentation of 12p DNA sequences were found in all cases. In addition, cryptic rearrangements of 12p were found in 39{\%} (7/18) of the i(12p)‐positive tumors and in 78{\%} (7/9) of the i(12p)‐negative tumors. Only 7{\%} (2/27) of all tumors had cryptic rearrangements of 12q.",
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AU - Smolarek, Teresa A.

AU - Blough, Ruthann I.

AU - Foster, Richard S.

AU - Ulbright, Thomas M.

AU - Palmer, Catherine G.

AU - Heerema, Nyla A.

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AB - The distribution of segments of the short and long arms of chromosome 12 was distinguished by two‐color fluorescence in situ hybridization (FISH) in 27 cytogenetically abnormal testicular germ cell tumors (TGCTs). A 12p‐specific probe was developed by chromosomal microdissection and sequence‐independent polymerase chain reaction (PCR) amplification and was combined with a commercially available whole‐chromosome 12 painting probe. The TGCTs included both i(12p)‐positive and i(12p)‐negative primary tumors and lymph node metastases from patients in clinical stage I or stage II who were not previously treated with chemotherapy. Rearrangements of the short arm of chromosome 12 and overrepresentation of 12p DNA sequences were found in all cases. In addition, cryptic rearrangements of 12p were found in 39% (7/18) of the i(12p)‐positive tumors and in 78% (7/9) of the i(12p)‐negative tumors. Only 7% (2/27) of all tumors had cryptic rearrangements of 12q.

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