Identification of novel matrix metalloproteinase-7 (matrilysin) cleavage sites in murine and human Fas ligand

Tracy Vargo-Gogola, Howard C. Crawford, Barbara Fingleton, Lynn M. Matrisian

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

Soluble Fas ligand (sFasL) is released from the cell surface by matrix metalloproteinases (MMPs), one of which is MMP-7. We have reported that MMP-7-generated sFasL is pro-apoptotic in both in vitro and in vivo systems. However, there are contradictory reports that the soluble form of FasL is inactive or anti-apoptotic, resulting in significant controversy in the literature. One potential explanation for these discrepancies is that forms of sFasL with different amino-terminal sequences have been demonstrated to have varying activities. Here we report that MMP-7 cleaves murine and human FasL at sites that are distinct from previously reported cleavage sites resulting in production of novel forms of sFasL. Cleavage of FasL by MMP-7 occurs at the leucine residues in the sequence "ELAELR" within the region between the transmembrane and trimerization domains. When this site is unavailable, a more c-terminal site, "SL," is cleaved. MMP-7 di4erentially processes murine and human FasL since it cleaves human FasL not only at the "ELAELR" site but also at a previously identified site. Additionally, MMP-3, but not MMP-2, was found to have the same cleavage specificity for murine FasL as MMP-7. We conclude that the controversy regarding the biological activity of sFasL may be explained, in part, by the generation of distinct forms of sFasL as a result of cleavage at specific sequences.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume408
Issue number2
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Keywords

  • Apoptosis
  • MMP-3
  • MMP-7
  • Proteolysis
  • Soluble FasL

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Identification of novel matrix metalloproteinase-7 (matrilysin) cleavage sites in murine and human Fas ligand'. Together they form a unique fingerprint.

  • Cite this